Stimulating effect of tuftsin and its analogues on the defective monocyte chemotaxis in systemic lupus erythematosus

Katalin Lukács, Gyöngyi Szabó, Ildikó Sonkoly, Éva Végh, János Gács, Mária Szekerke, Gyula Szegedi

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17 Citations (Scopus)

Abstract

Monocytes and macrophages are engaged at various levels of cellular immune reactivity. In addition to their function in the defensive mechanism directed at infective agents, they also play a basic role in immune complex elimination and antigen handling. Previous experiments revealed that systemic lupus erythematousus (SLE), the main representative of the autoimmune diseases, is associated with impaired monocyte chemotaxis. The endogenous basic tetrapeptide tufts in and 6 of its analogues were examined in vitro for their stimulating capacity of the chemotactic responsiveness of monocytes derived from patients with SLE. The monocyte migration assay was carried out by a modified Boyden technique and quantified by the leading front distance method and by counting the total distance covered by the monocyte locomotion. Tuftsin and 3 of its analogues significantly increased the defective chemotaxis in SLE. The tetrapeptides effective on chemotaxis also stimulated random migration and phagocytosis of the monocytes, albeit to a lesser extent. Structure-activity relationships, as well as the influence of the clinical stage of the disease were also examined. Experimental evidence leads to a favourable prediction for the immunotherapeutic value of these oligopeptides for the control of infections and the progression of the disease in patients with systemic lupus erythematosus.

Original languageEnglish
Pages (from-to)171-178
Number of pages8
JournalImmunopharmacology
Volume7
Issue number3-4
DOIs
Publication statusPublished - Jun 1984

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Keywords

  • Chemotaxis
  • Monocytes
  • Phagocytosis
  • Random migration
  • Systemic lupus erythematosus
  • Tetrapeptide analogues
  • Tuftsin

ASJC Scopus subject areas

  • Pharmacology

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