Stigmatellin probes the electrostatic potential in the QB site of the photosynthetic reaction center

László Gerencsér, Bogáta Boros, Valerie Derrien, Deborah K. Hanson, Colin A. Wraight, Pierre Sebban, P. Maróti

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The electrostatic potential in the secondary quinone (QB) binding site of the reaction center (RC) of the photosynthetic bacterium Rhodobacter sphaeroides determines the rate and free energy change (driving force) of electron transfer to QB. It is controlled by the ionization states of residues in a strongly interacting cluster around the QB site. Reduction of the QB induces change of the ionization states of residues and binding of protons from the bulk. Stigmatellin, an inhibitor of the mitochondrial and photosynthetic respiratory chain, has been proven to be a unique voltage probe of the QB binding pocket. It binds to the QB site with high affinity, and the pK value of its phenolic group monitors the local electrostatic potential with high sensitivity. Investigations with different types of detergent as a model system of isolated RC revealed that the pK of stigmatellin was controlled overwhelmingly by electrostatic and slightly by hydrophobic interactions. Measurements showed a high pK value (>11) of stigmatellin in the QB pocket of the dark-state wild-type RC, indicating substantial negative potential. When the local electrostatics of the QB site was modulated by a single mutation, L213Asp→Ala, or double mutations, L213Asp-L212Glu→Ala-Ala (AA), the pK of stigmatellin dropped to 7.5 and 7.4, respectively, which corresponds to a >210 mV increase in the electrostatic potential relative to the wild-type RC. This significant pK drop (ΔpK > 3.5) decreased dramatically to (ΔpK > 0.75) in the RC of the compensatory mutant (AA+M44Asn→AA+M44Asp). Our results indicate that the L213Asp is the most important actor in the control of the electrostatic potential in the QB site of the dark-state wild-type RC, in good accordance with conclusions of former studies using theoretical calculations or light-induced charge recombination assay.

Original languageEnglish
Pages (from-to)379-394
Number of pages16
JournalBiophysical Journal
Volume108
Issue number2
DOIs
Publication statusPublished - Jan 20 2015

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Photosynthetic Reaction Center Complex Proteins
Static Electricity
Rhodobacter sphaeroides
Mutation
Electron Transport
Hydrophobic and Hydrophilic Interactions
Detergents
Genetic Recombination
stigmatellin
Protons
Theoretical Models
Binding Sites
Electrons
Bacteria
Light

ASJC Scopus subject areas

  • Biophysics

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Stigmatellin probes the electrostatic potential in the QB site of the photosynthetic reaction center. / Gerencsér, László; Boros, Bogáta; Derrien, Valerie; Hanson, Deborah K.; Wraight, Colin A.; Sebban, Pierre; Maróti, P.

In: Biophysical Journal, Vol. 108, No. 2, 20.01.2015, p. 379-394.

Research output: Contribution to journalArticle

Gerencsér, László ; Boros, Bogáta ; Derrien, Valerie ; Hanson, Deborah K. ; Wraight, Colin A. ; Sebban, Pierre ; Maróti, P. / Stigmatellin probes the electrostatic potential in the QB site of the photosynthetic reaction center. In: Biophysical Journal. 2015 ; Vol. 108, No. 2. pp. 379-394.
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abstract = "The electrostatic potential in the secondary quinone (QB) binding site of the reaction center (RC) of the photosynthetic bacterium Rhodobacter sphaeroides determines the rate and free energy change (driving force) of electron transfer to QB. It is controlled by the ionization states of residues in a strongly interacting cluster around the QB site. Reduction of the QB induces change of the ionization states of residues and binding of protons from the bulk. Stigmatellin, an inhibitor of the mitochondrial and photosynthetic respiratory chain, has been proven to be a unique voltage probe of the QB binding pocket. It binds to the QB site with high affinity, and the pK value of its phenolic group monitors the local electrostatic potential with high sensitivity. Investigations with different types of detergent as a model system of isolated RC revealed that the pK of stigmatellin was controlled overwhelmingly by electrostatic and slightly by hydrophobic interactions. Measurements showed a high pK value (>11) of stigmatellin in the QB pocket of the dark-state wild-type RC, indicating substantial negative potential. When the local electrostatics of the QB site was modulated by a single mutation, L213Asp→Ala, or double mutations, L213Asp-L212Glu→Ala-Ala (AA), the pK of stigmatellin dropped to 7.5 and 7.4, respectively, which corresponds to a >210 mV increase in the electrostatic potential relative to the wild-type RC. This significant pK drop (ΔpK > 3.5) decreased dramatically to (ΔpK > 0.75) in the RC of the compensatory mutant (AA+M44Asn→AA+M44Asp). Our results indicate that the L213Asp is the most important actor in the control of the electrostatic potential in the QB site of the dark-state wild-type RC, in good accordance with conclusions of former studies using theoretical calculations or light-induced charge recombination assay.",
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