Steroid hormone (hydrocortisone, oestradiol and testosterone) uptake, storage or induced synthesis in Tetrahymena

G. Csaba, Eva Poteczin, T. Fehér, P. Kovács

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

After cyclodextrin-coated 10-6 M steroid hormone treatment for 3 days (hormonal imprinting), Tetrahymena cells and their media were analysed by radioimmunoassay for the same hormone and for the presence of the other two. In the absence of hormone treatment, the cells contained no detectable levels of the three steroids. By 2 days in fresh medium following exposure of cells to a 72 h pretreatment of each specific hormone, correspondingly high quantities of hydrocortisone and oestradiol, but lesser quantities of testosterone, were found in both the media and the cells. One week after treatment only traces of hydrocortisone were found, exclusively within the cells themselves. Oestradiol was present in measurable quantities in both cells and media, whereas testosterone was only present in the medium. The presence of the other two hormones to the one used in the pretreatment were not usually present, except that when testosterone had been given, some oestradiol was also detected at 48 h, suggesting Tetrahymena has a functional cytochrome P450 aromatase.

Original languageEnglish
Pages (from-to)875-878
Number of pages4
JournalCell Biology International
Volume22
Issue number11-12
DOIs
Publication statusPublished - Nov 1998

Fingerprint

Tetrahymena
Hydrocortisone
Testosterone
Estradiol
Steroids
Hormones
Aromatase
Cyclodextrins
Cytochrome P-450 Enzyme System
Radioimmunoassay

Keywords

  • Aromatase
  • Hormonal imprinting
  • Hormone induction
  • Steroid hormones
  • Tetrahymena

ASJC Scopus subject areas

  • Cell Biology

Cite this

Steroid hormone (hydrocortisone, oestradiol and testosterone) uptake, storage or induced synthesis in Tetrahymena. / Csaba, G.; Poteczin, Eva; Fehér, T.; Kovács, P.

In: Cell Biology International, Vol. 22, No. 11-12, 11.1998, p. 875-878.

Research output: Contribution to journalArticle

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