Steroid binding to feather follicles in the chicken

K. Kovács, P. Peczely, Z. Szelenyi

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Steroid binding profiles in chicken feather follicles were studied in vitro. Progesterone and promegestone were bound specifically with high affinity (dissociation constant = 7.79 nmol/l in females and 2.0 nmol/l in males) and with low capacity to the high-speed supernatant fraction (cytosol) of homogenized feather follicles. The number of progestin-binding sites was significantly (P <0.01) higher in females than in males (1.35 ± 0.07 (mean ± S.D.) vs 0.605 ± 0.15 pmol/mg protein). Significant cross-competition was observed between progestins and glucocorticoids for the binding sites. The heat-activated progestin-receptor complexes bound specifically to DNA-cellulose in vitro. The DNA-cellulose binding was 36.6% of the total specific binding. The presence of progestin-binding sites in the feather follicles implies a direct action of progesterone in this structure, which may be an important factor in the regulation of moulting. The lack of oestrogen binding to the cytosol prepared from the feather follicles suggests different regulation of progestin receptors in different target organs.

Original languageEnglish
Pages (from-to)187-191
Number of pages5
JournalJournal of Endocrinology
Volume109
Issue number2
Publication statusPublished - 1986

Fingerprint

Feathers
Chickens
Progestins
Steroids
Binding Sites
Progesterone Receptors
Cytosol
Progesterone
Promegestone
Molting
Glucocorticoids
Estrogens
Hot Temperature
Proteins
DNA-cellulose
In Vitro Techniques

ASJC Scopus subject areas

  • Endocrinology

Cite this

Kovács, K., Peczely, P., & Szelenyi, Z. (1986). Steroid binding to feather follicles in the chicken. Journal of Endocrinology, 109(2), 187-191.

Steroid binding to feather follicles in the chicken. / Kovács, K.; Peczely, P.; Szelenyi, Z.

In: Journal of Endocrinology, Vol. 109, No. 2, 1986, p. 187-191.

Research output: Contribution to journalArticle

Kovács, K, Peczely, P & Szelenyi, Z 1986, 'Steroid binding to feather follicles in the chicken', Journal of Endocrinology, vol. 109, no. 2, pp. 187-191.
Kovács, K. ; Peczely, P. ; Szelenyi, Z. / Steroid binding to feather follicles in the chicken. In: Journal of Endocrinology. 1986 ; Vol. 109, No. 2. pp. 187-191.
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