Stereoselective interaction of drug enantiomers with human serum transferrin in capillary zone electrophoresis (II)

Martin G. Schmid, Gerald Gübitz, F. Kilár

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Further studies on chiral resolution of drugs with different chemical structures by capillary zone electrophoresis using iron-free human serum transferrin are described. The substances passed a highly concentrated pseudo-stationary protein zone applied in a coated capillary and the possible chiral separation of the optical isomers was followed. Eighteen drugs with different structures were screened, and the enantiomers of clofedanol, buphenine, acebutolol and chlorphenamine were resolved. Several, but not all drugs, showed longer migration times while passing the protein zone, indicating an interaction with transferrin, although chiral resolution was not observed in all cases. The observations provided further information about the properties of the surface interaction sites of transferrin.

Original languageEnglish
Pages (from-to)282-287
Number of pages6
JournalElectrophoresis
Volume19
Issue number2
DOIs
Publication statusPublished - Feb 1998

Fingerprint

Enantiomers
Capillary Electrophoresis
Transferrin
Electrophoresis
Drug Interactions
Nylidrin
Serum
Acebutolol
Pharmaceutical Preparations
Chlorpheniramine
Surface Properties
Isomers
Proteins
Iron

Keywords

  • Capillary electrophoresis
  • Chiral separation
  • Drugs
  • Surface binding site
  • Transferrin

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Stereoselective interaction of drug enantiomers with human serum transferrin in capillary zone electrophoresis (II). / Schmid, Martin G.; Gübitz, Gerald; Kilár, F.

In: Electrophoresis, Vol. 19, No. 2, 02.1998, p. 282-287.

Research output: Contribution to journalArticle

@article{1ceae7d10d7d4e35976ab6d2eb8b63d5,
title = "Stereoselective interaction of drug enantiomers with human serum transferrin in capillary zone electrophoresis (II)",
abstract = "Further studies on chiral resolution of drugs with different chemical structures by capillary zone electrophoresis using iron-free human serum transferrin are described. The substances passed a highly concentrated pseudo-stationary protein zone applied in a coated capillary and the possible chiral separation of the optical isomers was followed. Eighteen drugs with different structures were screened, and the enantiomers of clofedanol, buphenine, acebutolol and chlorphenamine were resolved. Several, but not all drugs, showed longer migration times while passing the protein zone, indicating an interaction with transferrin, although chiral resolution was not observed in all cases. The observations provided further information about the properties of the surface interaction sites of transferrin.",
keywords = "Capillary electrophoresis, Chiral separation, Drugs, Surface binding site, Transferrin",
author = "Schmid, {Martin G.} and Gerald G{\"u}bitz and F. Kil{\'a}r",
year = "1998",
month = "2",
doi = "10.1002/elps.1150190223",
language = "English",
volume = "19",
pages = "282--287",
journal = "Electrophoresis",
issn = "0173-0835",
publisher = "Wiley-VCH Verlag",
number = "2",

}

TY - JOUR

T1 - Stereoselective interaction of drug enantiomers with human serum transferrin in capillary zone electrophoresis (II)

AU - Schmid, Martin G.

AU - Gübitz, Gerald

AU - Kilár, F.

PY - 1998/2

Y1 - 1998/2

N2 - Further studies on chiral resolution of drugs with different chemical structures by capillary zone electrophoresis using iron-free human serum transferrin are described. The substances passed a highly concentrated pseudo-stationary protein zone applied in a coated capillary and the possible chiral separation of the optical isomers was followed. Eighteen drugs with different structures were screened, and the enantiomers of clofedanol, buphenine, acebutolol and chlorphenamine were resolved. Several, but not all drugs, showed longer migration times while passing the protein zone, indicating an interaction with transferrin, although chiral resolution was not observed in all cases. The observations provided further information about the properties of the surface interaction sites of transferrin.

AB - Further studies on chiral resolution of drugs with different chemical structures by capillary zone electrophoresis using iron-free human serum transferrin are described. The substances passed a highly concentrated pseudo-stationary protein zone applied in a coated capillary and the possible chiral separation of the optical isomers was followed. Eighteen drugs with different structures were screened, and the enantiomers of clofedanol, buphenine, acebutolol and chlorphenamine were resolved. Several, but not all drugs, showed longer migration times while passing the protein zone, indicating an interaction with transferrin, although chiral resolution was not observed in all cases. The observations provided further information about the properties of the surface interaction sites of transferrin.

KW - Capillary electrophoresis

KW - Chiral separation

KW - Drugs

KW - Surface binding site

KW - Transferrin

UR - http://www.scopus.com/inward/record.url?scp=0345698821&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0345698821&partnerID=8YFLogxK

U2 - 10.1002/elps.1150190223

DO - 10.1002/elps.1150190223

M3 - Article

C2 - 9548292

AN - SCOPUS:0345698821

VL - 19

SP - 282

EP - 287

JO - Electrophoresis

JF - Electrophoresis

SN - 0173-0835

IS - 2

ER -