A new efficient OPLC method has been developed for the separation of seven biogenic amines (agmatine, spermine, spermidine, putrescine, cadaverine, histamine, tyramine, and NH3 as interfering component) in foods. The separation of the dansylated derivatives of the amines was performed on 20 cm x 20 cm silica gel HPTLC plates with stepwise gradient elution using the Personal OPLC BS 50 overpressured-layer chromatograph. The first solvent system was n-hexane-n-butanol-triethylamine, 90 + 10 + 9.1 (v/v) and the second was n-hexane-n-butanol, 8 + 2 (v/v). Approximately 26 min were needed for simultaneous separation of 14 samples; the detection limit was 2.5-15 ng spot-1, approximately 100 times lower than the critical toxic limit in foods. The method has been used successfully to screen for biogenic amines in samples of different vegetables.
|Number of pages||4|
|Journal||Journal of Planar Chromatography - Modern TLC|
|Publication status||Published - Jan 1 1998|
- Biogenic amine
ASJC Scopus subject areas
- Analytical Chemistry
- Clinical Biochemistry