Steady-state fluorescence quenching applications for studying protein structure and dynamics

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90 Citations (Scopus)


Fluorescence quenching methods are useful to obtain information about the conformational and/or dynamic changes of proteins in complex macromolecular systems. In this review steady-state methods are described and the data interpretation is thoroughly discussed. As a special case of fluorescence quenching mechanism, fluorescence resonance energy transfer (FRET) phenomenon is also presented. Application of a FRET based method to characterize the temperature dependence of the flexibility of protein matrix is clearly demonstrated.

Original languageEnglish
Pages (from-to)223-236
Number of pages14
JournalJournal of Photochemistry and Photobiology B: Biology
Issue number3
Publication statusPublished - Jun 1 2006


  • FRET
  • Fluorescence quenching
  • Protein structure
  • Tryptophan fluorescence

ASJC Scopus subject areas

  • Radiation
  • Radiological and Ultrasound Technology
  • Biophysics
  • Radiology Nuclear Medicine and imaging

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