The continuous exchange of the radioactive free ligand by its non-labelled isotopomer of identical concentration does not affect the position of the overall-equilibrium. The replacement of the enantiomer of higher binding affinity lags behind the replacement of the other one that leads to stereoselective labelling of racemates. Radioactivity, as a function of filtrate volume can be fitted to theoretical expressions. The method allows to study stereoselectivity of binding equilibria set by racemic ligands.
ASJC Scopus subject areas
- Molecular Biology