State-of-the-art enantioseparations of natural and unnatural amino acids by high-performance liquid chromatography

István Ilisz, Antal Péter, Wolfgang Lindner

Research output: Contribution to journalReview article

42 Citations (Scopus)


This review discusses recent publications on the separation and analysis of natural and unnatural amino acid enantiomers by liquid chromatography. Focus is placed on methodological aspects relating chiral stationary phases and chiral columns which can cope with the challenge. Conceptually, amino acids can be enantioseparated in free form, which refers to the resolution of polar ampholytes, or as N-protected amino acids, which can be regarded as acidic commodities. Such synthons are used, for instance, in peptide synthesis protocols. Amino groups are frequently tagged with highly fluorescent or MS/MS active labels in order to generate sensitive and simultaneously stereoselective assays of diverse amino acids in complex matrices. It is our intention to present the state of the art of enantioselective amino acid analysis by HPLC concepts and to pinpoint practical aspects.

Original languageEnglish
Pages (from-to)11-22
Number of pages12
JournalTrAC - Trends in Analytical Chemistry
Publication statusPublished - Jul 1 2016



  • Chiral stationary phases
  • Enantioseparation
  • High-performance liquid chromatography
  • N-tagged amino acids
  • α-, β- and γ-amino acids

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy

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