Starvation- and xenobiotic-related transcriptomic responses of the sulfanilic acid-degrading bacterium, Novosphingobium resinovorum SA1

Botond Hegedüs, P. Kós, Gábor Bende, Naila Bounedjoum, Gergely Maróti, Krisztián Laczi, Márk Szuhaj, Katalin Perei, G. Rákhely

Research output: Contribution to journalArticle

Abstract

Novosphingobium resinovorum SA1 was the first single isolate capable of degrading sulfanilic acid, a widely used representative of sulfonated aromatic compounds. The genome of the strain was recently sequenced, and here, we present whole-cell transcriptome analyses of cells exposed to sulfanilic acid as compared to cells grown on glucose. The comparison of the transcript profiles suggested that the primary impact of sulfanilic acid on the cell transcriptome was a starvation-like effect. The genes of the peripheral, central, and common pathways of sulfanilic acid biodegradation had distinct transcript profiles. The peripheral genes located on a plasmid had very high basal expressions which were hardly upregulated by sulfanilic acid. The genomic context and the codon usage preference of these genes suggested that they were acquired by horizontal gene transfer. The genes of the central pathways were remarkably inducible by sulfanilic acid indicating the presence of a substrate-specific regulatory system in the cells. Surprisingly, the genes of the common part of the metabolic pathway had low and sulfanilic acid-independent transcript levels. The approach applied resulted in the identification of the genes of proteins involved in auxiliary processes such as electron transfer, substrate and iron transports, sulfite oxidases, and sulfite transporters. The whole transcriptome analysis revealed that the cells exposed to xenobiotics had multiple responses including general starvation-like, substrate-specific, and substrate-related effects. From the results, we propose that the genes of the peripheral, central, and common parts of the pathway have been evolved independently.

Original languageEnglish
Pages (from-to)305-318
Number of pages14
JournalApplied Microbiology and Biotechnology
Volume102
Issue number1
DOIs
Publication statusPublished - Jan 1 2018

Fingerprint

Sulfanilic Acids
Xenobiotics
Starvation
Bacteria
Genes
Gene Expression Profiling
Oxidoreductases Acting on Sulfur Group Donors
Horizontal Gene Transfer
Sulfites
Metabolic Networks and Pathways
Transcriptome
Codon
Plasmids
Iron
Genome
Electrons
Glucose

Keywords

  • Metabolic response
  • Novosphingobium
  • Starvation
  • Sulfonated aromatic acids
  • Transcriptomics

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Starvation- and xenobiotic-related transcriptomic responses of the sulfanilic acid-degrading bacterium, Novosphingobium resinovorum SA1. / Hegedüs, Botond; Kós, P.; Bende, Gábor; Bounedjoum, Naila; Maróti, Gergely; Laczi, Krisztián; Szuhaj, Márk; Perei, Katalin; Rákhely, G.

In: Applied Microbiology and Biotechnology, Vol. 102, No. 1, 01.01.2018, p. 305-318.

Research output: Contribution to journalArticle

Hegedüs, Botond ; Kós, P. ; Bende, Gábor ; Bounedjoum, Naila ; Maróti, Gergely ; Laczi, Krisztián ; Szuhaj, Márk ; Perei, Katalin ; Rákhely, G. / Starvation- and xenobiotic-related transcriptomic responses of the sulfanilic acid-degrading bacterium, Novosphingobium resinovorum SA1. In: Applied Microbiology and Biotechnology. 2018 ; Vol. 102, No. 1. pp. 305-318.
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AB - Novosphingobium resinovorum SA1 was the first single isolate capable of degrading sulfanilic acid, a widely used representative of sulfonated aromatic compounds. The genome of the strain was recently sequenced, and here, we present whole-cell transcriptome analyses of cells exposed to sulfanilic acid as compared to cells grown on glucose. The comparison of the transcript profiles suggested that the primary impact of sulfanilic acid on the cell transcriptome was a starvation-like effect. The genes of the peripheral, central, and common pathways of sulfanilic acid biodegradation had distinct transcript profiles. The peripheral genes located on a plasmid had very high basal expressions which were hardly upregulated by sulfanilic acid. The genomic context and the codon usage preference of these genes suggested that they were acquired by horizontal gene transfer. The genes of the central pathways were remarkably inducible by sulfanilic acid indicating the presence of a substrate-specific regulatory system in the cells. Surprisingly, the genes of the common part of the metabolic pathway had low and sulfanilic acid-independent transcript levels. The approach applied resulted in the identification of the genes of proteins involved in auxiliary processes such as electron transfer, substrate and iron transports, sulfite oxidases, and sulfite transporters. The whole transcriptome analysis revealed that the cells exposed to xenobiotics had multiple responses including general starvation-like, substrate-specific, and substrate-related effects. From the results, we propose that the genes of the peripheral, central, and common parts of the pathway have been evolved independently.

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