Staphylococcus aureus bound to complement receptor 1 on human erythrocytes by bispecific monoclonal antibodies is phagocytosed by acceptor macrophages

Edit Gyimesi, Alexander J. Bankovich, Theodore A. Schuman, Joanna B. Goldberg, Margaret A. Lindorfer, Ronald P. Taylor

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Antibiotic resistant strains of Staphylococcus aureus pose a growing threat to public health, and immunotherapy offers potential modalities to combat antibiotic resistance. We prepared bispecific monoclonal antibody complexes (heteropolymers, HP), specific for the primate erythrocyte complement C3b receptor (CR1) and type 5 capsular polysaccharide of the T5 isolate of S. aureus. Fluorescence microscopy and flow cytometry revealed that HP promote binding of S. aureus to human erythrocytes. Incubation of erythrocyte-bound, HP-opsonized S. aureus with human monocyte/macrophages or mouse macrophage cell lines led to transfer, internalization and killing of bacteria by macrophages with little erythrocyte loss. This reaction is similar to the process in which C3b-opsonized substrates, bound to erythrocyte CR1 by immune adherence, are transferred to acceptor phagocytes. Our results provide the basis for development of an in vivo paradigm focused on immunotherapeutic approaches for treatment of infections due to antibiotic resistant bacteria.

Original languageEnglish
Pages (from-to)185-192
Number of pages8
JournalImmunology letters
Volume95
Issue number2
DOIs
Publication statusPublished - Sep 1 2004

Keywords

  • Erythrocyte CR1
  • Immune adherence
  • Staphylococcus aureus

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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