Standardization of the filter paper activity assay for solid substrate fermentation

Katalin Urbánszki, György Szakács, Robert P. Tengerdy

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The conditions of the filter paper activity (FPA) assay were standardized for solid substrate fermentation (SSF). The FPA is a relative measure of the overall cellulose hydrolysing capacity of microbial cellulase preparations, thus reliable and comparable data may be obtained only under standardized conditions. The standardization developed for submerged fermentation (SF) cannot be translated directly to SSF. In SSF, the FPA is strongly dependent on the extraction volume and on the dilution of the enzyme in the assay. The optimal extraction volume was substrate dependent in SSF of corn fiber, spent brewing grains and wheat straw for cellulase production by Trichoderma reesei Rut C30. Other cellulolytic enzyme assays (endoglucanase, beta-glucosidase and xylanase) were much less sensitive to the extraction volume.

Original languageEnglish
Pages (from-to)65-69
Number of pages5
JournalBiotechnology Letters
Volume22
Issue number1
DOIs
Publication statusPublished - Jan 1 2000

Keywords

  • Cellulase production by solid substrate fermentation
  • Enzyme extraction
  • Filter paper activity
  • Trichoderma reesei Rut C30

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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