Stage-related superoxide anion production of granulocytes of gynecologic cancer patients

Robert Póka, Sándor Szûcs, Róza Ádány, Edit Szikszay

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Objective: To measure superoxide anion production of polymorphonuclear leukocytes (PMNLs) in 58 gynecologic cancer patients and compare to that of healthy controls. Methods: PMNLs were separated from peripheral blood samples by Ficoll and subsequent Percoll gradient sedimentation. Baseline and phorbol-dibutyrate (100 nmol/l) stimulated superoxide anion production was measured spectrophotometrically as superoxide dismutase inhibitable reduction of ferricytochrome c (50 μmol/l) absorbance. Differences between the mean superoxide anion production of different patient groups and the control group were assessed by Student's t-test. Results: The mean superoxide anion production of PMNLs of healthy controls was 1.855 nM/min/3·10 5 cells (SD=0.211 nM/min/3·10 5 cells). Superoxide anion production of gynecologic cancer patients and healthy controls varied in a wide range. PMNLs of patients had lower baseline and stimulated activity than those of healthy volunteers. The frequency of a mean superoxide production at least 2xSD below the control showed a parallel increase with advancing stage. Conclusion: Granulocytes of gynecologic cancer patients have reduced capacity and inducibility of superoxide anion production already at an early stage of disease. (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)55-57
Number of pages3
JournalEuropean Journal of Obstetrics and Gynecology and Reproductive Biology
Volume89
Issue number1
DOIs
Publication statusPublished - Mar 2000

Keywords

  • Granulocyte
  • Gynecologic cancer
  • Superoxide anion

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynaecology

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