Stable production of human insulin-like growth factor 1 (IGF-1) in the milk of hemi- and homozygous transgenic rabbits over several generations

Natascha Zinovieva, Caroline Lassnig, Dieter Schams, Urban Besenfelder, Eckhard Wolf, Sigrid Müller, L. Frenyó, Janos Seregi, Mathias Müller, Gottfried Brem

Research output: Contribution to journalArticle

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Abstract

One transgenic rabbit line was generated carrying a fusion gene consisting of the cDNA for human IGF-1 fused to a mammary gland specific expression cassette derived from bovine alpha-S1-casein sequences. Transgene expression was shown to be strictly tissue and lactation period specific. The transgenic rabbit line was bred for six generations. All transgenic animals showed stable production of biologically active IGF-1 over the generations and no apparent effect on the physiological or reproductive performance was observed. The absence of adverse effects on homozygous transgenic rabbits suggested the absence of insertional mutagenesis. Eight hemizygous transgenic offspring analysed produced on average 363 ± 12 μg/ml (ranging from 223 ± 61 to 484 ± 39 μg/ml) mature human IGF-1 in their milk, whereas three homozygous animals produced on average 543 ± 41 μg/ml (ranging from 360 ± 15 to 678 ± 80 μg/ml). Homozygous huIGF-1 females clearly showed a significantly increased production performance of the recombinant protein.

Original languageEnglish
Pages (from-to)437-447
Number of pages11
JournalTransgenic Research
Volume7
Issue number6
DOIs
Publication statusPublished - Nov 1998

Fingerprint

somatomedins
Somatomedins
Milk
rabbits
genetically modified organisms
Rabbits
milk
Genetically Modified Animals
Insertional Mutagenesis
Gene Fusion
Human Mammary Glands
alphaS1-casein
Caseins
insertional mutagenesis
Transgenes
Lactation
Recombinant Proteins
transgenic animals
gene fusion
Complementary DNA

Keywords

  • Bioreactor
  • Gene farming
  • Genetic engineering
  • Mammary gland
  • Milk composition
  • Recombinant protein

ASJC Scopus subject areas

  • Genetics
  • Plant Science
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Biotechnology
  • Food Science

Cite this

Stable production of human insulin-like growth factor 1 (IGF-1) in the milk of hemi- and homozygous transgenic rabbits over several generations. / Zinovieva, Natascha; Lassnig, Caroline; Schams, Dieter; Besenfelder, Urban; Wolf, Eckhard; Müller, Sigrid; Frenyó, L.; Seregi, Janos; Müller, Mathias; Brem, Gottfried.

In: Transgenic Research, Vol. 7, No. 6, 11.1998, p. 437-447.

Research output: Contribution to journalArticle

Zinovieva, N, Lassnig, C, Schams, D, Besenfelder, U, Wolf, E, Müller, S, Frenyó, L, Seregi, J, Müller, M & Brem, G 1998, 'Stable production of human insulin-like growth factor 1 (IGF-1) in the milk of hemi- and homozygous transgenic rabbits over several generations', Transgenic Research, vol. 7, no. 6, pp. 437-447. https://doi.org/10.1023/A:1008831028620
Zinovieva, Natascha ; Lassnig, Caroline ; Schams, Dieter ; Besenfelder, Urban ; Wolf, Eckhard ; Müller, Sigrid ; Frenyó, L. ; Seregi, Janos ; Müller, Mathias ; Brem, Gottfried. / Stable production of human insulin-like growth factor 1 (IGF-1) in the milk of hemi- and homozygous transgenic rabbits over several generations. In: Transgenic Research. 1998 ; Vol. 7, No. 6. pp. 437-447.
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