Stable isotope dynamic labeling of secretomes (SIDLs) identifies authentic secretory proteins released by cancer and stromal cells

Dean E. Hammond, J. Dinesh Kumar, Lorna Raymond, Deborah M. Simpson, Robert J. Beynon, Graham J. Dockray, A. Varró

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Analysis of secretomes critically underpins the capacity to understand the mechanisms determining interactions between cells and between cells and their environment. In the context of cancer cell micro-environments, the relevant interactions are recognized to be an important determinant of tumor progression. Global proteomic analyses of secretomes are often performed at a single time point and frequently identify both classical secreted proteins (possessing an N-terminal signal sequence), as well as many intracellular proteins, the release of which is of uncertain biological significance. Here, we describe a mass spectrometry-based method for stable isotope dynamic labeling of secretomes (SIDLS) that, by dynamic SILAC, discriminates the secretion kinetics of classical secretory proteins and intracellular proteins released from cancer and stromal cells in culture. SIDLS is a robust classifier of the different cellular origins of proteins within the secretome and should be broadly applicable to nonproliferating cells and cells grown in short term culture.

Original languageEnglish
Pages (from-to)1837-1849
Number of pages13
JournalMolecular and Cellular Proteomics
Volume17
Issue number9
DOIs
Publication statusPublished - Sep 1 2018

Fingerprint

Isotope Labeling
Stromal Cells
Isotopes
Labeling
Neoplasms
Proteins
Cells
Protein Sorting Signals
Cell culture
Cell Communication
Proteomics
Mass spectrometry
Tumors
Mass Spectrometry
Classifiers
Cell Culture Techniques
Kinetics

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Molecular Biology

Cite this

Stable isotope dynamic labeling of secretomes (SIDLs) identifies authentic secretory proteins released by cancer and stromal cells. / Hammond, Dean E.; Kumar, J. Dinesh; Raymond, Lorna; Simpson, Deborah M.; Beynon, Robert J.; Dockray, Graham J.; Varró, A.

In: Molecular and Cellular Proteomics, Vol. 17, No. 9, 01.09.2018, p. 1837-1849.

Research output: Contribution to journalArticle

Hammond, Dean E. ; Kumar, J. Dinesh ; Raymond, Lorna ; Simpson, Deborah M. ; Beynon, Robert J. ; Dockray, Graham J. ; Varró, A. / Stable isotope dynamic labeling of secretomes (SIDLs) identifies authentic secretory proteins released by cancer and stromal cells. In: Molecular and Cellular Proteomics. 2018 ; Vol. 17, No. 9. pp. 1837-1849.
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