Stable incorporation of genetic material into the chromosome of Rhizobium meliloti 41

construction of an integrative vector system

E. Hermesz, F. Olasz, László Dorgai, L. Orosz

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

An integrative vector system has been developed from the site-specific recombination elements of temperate phage 16-3. The system can be used for highly efficient stable introduction of genetic material into the chromosome of the symbiotic nitrogen-fixing organism, Rhizobium meliloti 41 (Rm41) at the attB site. Vectors carrying the phage-bome attachment site were constructed, and helper phages providing the site-specific recombination functions in trans were isolated. Other possible applications of the system are discussed.

Original languageEnglish
Pages (from-to)9-15
Number of pages7
JournalGene
Volume119
Issue number1
DOIs
Publication statusPublished - Sep 21 1992

Fingerprint

Sinorhizobium meliloti
Bacteriophages
Genetic Recombination
Microbiological Attachment Sites
Chromosomes
Genes
Nitrogen

Keywords

  • attachment sites
  • Int and Xis functions
  • molecular cloning
  • Recombinant DNA
  • site-specific recombination
  • temperate phage 16-3

ASJC Scopus subject areas

  • Genetics

Cite this

Stable incorporation of genetic material into the chromosome of Rhizobium meliloti 41 : construction of an integrative vector system. / Hermesz, E.; Olasz, F.; Dorgai, László; Orosz, L.

In: Gene, Vol. 119, No. 1, 21.09.1992, p. 9-15.

Research output: Contribution to journalArticle

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