Stability of sunflower 2S albumins and LTP to physiologically relevant in vitro gastrointestinal digestion

Bernadett Berecz, E. N. Clare Mills, István Parádi, Ferenc Láng, L. Tamás, Peter R. Shewry, Alan R. MacKie

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

In order for a protein to elicit a systemic allergic response it must reach the circulatory system through the intestinal mucosa as a sufficiently large fragment with adequate structural integrity. Sunflower LTP and 2S albumins (SFA8 and three mixed fractions of Alb1 and Alb2) were digested in simulated gastric fluid (SGF) for 2 h and the conditions were then changed to mimic the intestinal environment for a further 2 h digestion. The effects of phosphatidylcholine (PC) and emulsification on the digestibility of the proteins were investigated. PC protected all of the proteins studied against both gastric and intestinal digestive enzymes but to different extents. Emulsification of SFA8 resulted in strong protection against digestion, which was further enhanced by the presence of PC in the SGF. These results highlight the importance of considering real food structures such as emulsified systems and also the gastrointestinal environment that proteins are exposed to once consumed when assessing allergenicity.

Original languageEnglish
Pages (from-to)2374-2381
Number of pages8
JournalFood Chemistry
Volume138
Issue number4
DOIs
Publication statusPublished - Jun 15 2013

Fingerprint

Helianthus
phosphatidylcholines
Helianthus annuus
albumins
Digestion
Albumins
stomach
digestion
Phosphatidylcholines
emulsifying
Stomach
Emulsification
circulatory system
allergenicity
Proteins
proteins
digestive enzymes
intestinal mucosa
digestible protein
Fluids

Keywords

  • 2S albumins
  • Food allergy
  • Helianthus annuus L.
  • In vitro digestion
  • nsLTP
  • SFA8
  • Sunflower

ASJC Scopus subject areas

  • Food Science
  • Analytical Chemistry

Cite this

Stability of sunflower 2S albumins and LTP to physiologically relevant in vitro gastrointestinal digestion. / Berecz, Bernadett; Clare Mills, E. N.; Parádi, István; Láng, Ferenc; Tamás, L.; Shewry, Peter R.; MacKie, Alan R.

In: Food Chemistry, Vol. 138, No. 4, 15.06.2013, p. 2374-2381.

Research output: Contribution to journalArticle

Berecz, B, Clare Mills, EN, Parádi, I, Láng, F, Tamás, L, Shewry, PR & MacKie, AR 2013, 'Stability of sunflower 2S albumins and LTP to physiologically relevant in vitro gastrointestinal digestion', Food Chemistry, vol. 138, no. 4, pp. 2374-2381. https://doi.org/10.1016/j.foodchem.2012.12.034
Berecz, Bernadett ; Clare Mills, E. N. ; Parádi, István ; Láng, Ferenc ; Tamás, L. ; Shewry, Peter R. ; MacKie, Alan R. / Stability of sunflower 2S albumins and LTP to physiologically relevant in vitro gastrointestinal digestion. In: Food Chemistry. 2013 ; Vol. 138, No. 4. pp. 2374-2381.
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