(s4dU)35: A novel, highly potent oligonucleotide inhibitor of the human immunodeficiency virus type 1 reverse transcriptase

Szilvia Tokés, Janos Aradi

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Oligodeoxycytidylates were converted to s4dUMP-containing oligomers by treatment with liquid H2S. The inhibitory potency of the modified oligonucleotides on human immunodeficiency virus type 1 reverse transcriptase depended on the chain length and on the percentage of modification. The most potent reverse transcriptase inhibitor was (s4dU)35. The inhibitory pattern was competitive, when either poly(A)·(dT)16 or poly(C)·(dG)16 was used as template-primer (variable substrate), suggesting that the free enzyme interacts with (s4dU)35. The K(i) values were 3.0 and 2.2 nM in the presence of poly(A)·(dT)16 and poly(C)·(dG)16, respectively.

Original languageEnglish
Pages (from-to)43-46
Number of pages4
JournalFEBS letters
Volume396
Issue number1
DOIs
Publication statusPublished - Oct 28 1996

Keywords

  • Antitemplate
  • Human immunodeficiency virus
  • Inhibition
  • Oligo 4-thio-2'-deoxyuridylate
  • Reverse transcriptase

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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