SSR markers as tools in maize breeding for high starch content

E. Nagy, I. Timár, Z. Hegyi, T. Spitkó, L. Márton

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The chemical composition of maize makes it suitable for a wide range of non-conventional uses, including utilisation as a new source of energy for the 21 st century as a raw material for biofuel. The aim of the experiments was to amalgamate the application of genetic markers with conventional breeding methods to produce maize hybrids whose starch content and ecostability satisfied the demands of industrial use, while having yield potential and agronomic traits on a par with those of hybrids currently cultivated. The chemical quality of 220 lines was evaluated using the NIR spectrometric technique, and the five maize inbred lines with the lowest and highest starch contents were selected for genetic marker studies. The variety identification of the lines was carried out using the isoenzymes stipulated by UPOV. The following SSR markers were tested: phi095, umcl057, nc004, phi096, nc007, umcl564, phi85, ylSSR, umc1178, nc009, phi070, umcl066, umcl74l, umcl069, phi033, phi06l, wx, phi032, phi084 and phi062. The analysis of the fragment patterns revealed three SSR markers that appeared to be correlated with the starch content of the maize lines. These were the primer pairs ylSSR, umcl069 and phi062. These results are only of a preliminary nature, however, as the incorporation of starch is probably regulated by several genes, and the studies suggest it is also influenced by several environmental factors. It also appears likely that the bioethanol yield is determined not only by the starch content, but also by other parameters. Further research should thus be expanded to include investigations into the structural and fermentability traits of starch molecules, including the characterisation of these traits using genetic markers.

Original languageEnglish
Pages (from-to)253-257
Number of pages5
JournalMaydica
Volume54
Issue number2-3
Publication statusPublished - 2009

Fingerprint

Starch
Zea mays
Breeding
starch
corn
breeding
Genetic Markers
genetic markers
bioethanol
Biofuels
breeding methods
biofuels
agronomic traits
inbred lines
Isoenzymes
raw materials
isozymes
chemical composition
environmental factors
energy

Keywords

  • Bioethanol
  • Maize
  • Nir spectroscopy
  • SSR markers
  • Starch content

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

Cite this

Nagy, E., Timár, I., Hegyi, Z., Spitkó, T., & Márton, L. (2009). SSR markers as tools in maize breeding for high starch content. Maydica, 54(2-3), 253-257.

SSR markers as tools in maize breeding for high starch content. / Nagy, E.; Timár, I.; Hegyi, Z.; Spitkó, T.; Márton, L.

In: Maydica, Vol. 54, No. 2-3, 2009, p. 253-257.

Research output: Contribution to journalArticle

Nagy, E, Timár, I, Hegyi, Z, Spitkó, T & Márton, L 2009, 'SSR markers as tools in maize breeding for high starch content', Maydica, vol. 54, no. 2-3, pp. 253-257.
Nagy E, Timár I, Hegyi Z, Spitkó T, Márton L. SSR markers as tools in maize breeding for high starch content. Maydica. 2009;54(2-3):253-257.
Nagy, E. ; Timár, I. ; Hegyi, Z. ; Spitkó, T. ; Márton, L. / SSR markers as tools in maize breeding for high starch content. In: Maydica. 2009 ; Vol. 54, No. 2-3. pp. 253-257.
@article{3a0c559da2a44678b456dd45de282740,
title = "SSR markers as tools in maize breeding for high starch content",
abstract = "The chemical composition of maize makes it suitable for a wide range of non-conventional uses, including utilisation as a new source of energy for the 21 st century as a raw material for biofuel. The aim of the experiments was to amalgamate the application of genetic markers with conventional breeding methods to produce maize hybrids whose starch content and ecostability satisfied the demands of industrial use, while having yield potential and agronomic traits on a par with those of hybrids currently cultivated. The chemical quality of 220 lines was evaluated using the NIR spectrometric technique, and the five maize inbred lines with the lowest and highest starch contents were selected for genetic marker studies. The variety identification of the lines was carried out using the isoenzymes stipulated by UPOV. The following SSR markers were tested: phi095, umcl057, nc004, phi096, nc007, umcl564, phi85, ylSSR, umc1178, nc009, phi070, umcl066, umcl74l, umcl069, phi033, phi06l, wx, phi032, phi084 and phi062. The analysis of the fragment patterns revealed three SSR markers that appeared to be correlated with the starch content of the maize lines. These were the primer pairs ylSSR, umcl069 and phi062. These results are only of a preliminary nature, however, as the incorporation of starch is probably regulated by several genes, and the studies suggest it is also influenced by several environmental factors. It also appears likely that the bioethanol yield is determined not only by the starch content, but also by other parameters. Further research should thus be expanded to include investigations into the structural and fermentability traits of starch molecules, including the characterisation of these traits using genetic markers.",
keywords = "Bioethanol, Maize, Nir spectroscopy, SSR markers, Starch content",
author = "E. Nagy and I. Tim{\'a}r and Z. Hegyi and T. Spitk{\'o} and L. M{\'a}rton",
year = "2009",
language = "English",
volume = "54",
pages = "253--257",
journal = "Maydica",
issn = "0025-6153",
publisher = "Experimental Institute for Cereal Research",
number = "2-3",

}

TY - JOUR

T1 - SSR markers as tools in maize breeding for high starch content

AU - Nagy, E.

AU - Timár, I.

AU - Hegyi, Z.

AU - Spitkó, T.

AU - Márton, L.

PY - 2009

Y1 - 2009

N2 - The chemical composition of maize makes it suitable for a wide range of non-conventional uses, including utilisation as a new source of energy for the 21 st century as a raw material for biofuel. The aim of the experiments was to amalgamate the application of genetic markers with conventional breeding methods to produce maize hybrids whose starch content and ecostability satisfied the demands of industrial use, while having yield potential and agronomic traits on a par with those of hybrids currently cultivated. The chemical quality of 220 lines was evaluated using the NIR spectrometric technique, and the five maize inbred lines with the lowest and highest starch contents were selected for genetic marker studies. The variety identification of the lines was carried out using the isoenzymes stipulated by UPOV. The following SSR markers were tested: phi095, umcl057, nc004, phi096, nc007, umcl564, phi85, ylSSR, umc1178, nc009, phi070, umcl066, umcl74l, umcl069, phi033, phi06l, wx, phi032, phi084 and phi062. The analysis of the fragment patterns revealed three SSR markers that appeared to be correlated with the starch content of the maize lines. These were the primer pairs ylSSR, umcl069 and phi062. These results are only of a preliminary nature, however, as the incorporation of starch is probably regulated by several genes, and the studies suggest it is also influenced by several environmental factors. It also appears likely that the bioethanol yield is determined not only by the starch content, but also by other parameters. Further research should thus be expanded to include investigations into the structural and fermentability traits of starch molecules, including the characterisation of these traits using genetic markers.

AB - The chemical composition of maize makes it suitable for a wide range of non-conventional uses, including utilisation as a new source of energy for the 21 st century as a raw material for biofuel. The aim of the experiments was to amalgamate the application of genetic markers with conventional breeding methods to produce maize hybrids whose starch content and ecostability satisfied the demands of industrial use, while having yield potential and agronomic traits on a par with those of hybrids currently cultivated. The chemical quality of 220 lines was evaluated using the NIR spectrometric technique, and the five maize inbred lines with the lowest and highest starch contents were selected for genetic marker studies. The variety identification of the lines was carried out using the isoenzymes stipulated by UPOV. The following SSR markers were tested: phi095, umcl057, nc004, phi096, nc007, umcl564, phi85, ylSSR, umc1178, nc009, phi070, umcl066, umcl74l, umcl069, phi033, phi06l, wx, phi032, phi084 and phi062. The analysis of the fragment patterns revealed three SSR markers that appeared to be correlated with the starch content of the maize lines. These were the primer pairs ylSSR, umcl069 and phi062. These results are only of a preliminary nature, however, as the incorporation of starch is probably regulated by several genes, and the studies suggest it is also influenced by several environmental factors. It also appears likely that the bioethanol yield is determined not only by the starch content, but also by other parameters. Further research should thus be expanded to include investigations into the structural and fermentability traits of starch molecules, including the characterisation of these traits using genetic markers.

KW - Bioethanol

KW - Maize

KW - Nir spectroscopy

KW - SSR markers

KW - Starch content

UR - http://www.scopus.com/inward/record.url?scp=76249100527&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=76249100527&partnerID=8YFLogxK

M3 - Article

VL - 54

SP - 253

EP - 257

JO - Maydica

JF - Maydica

SN - 0025-6153

IS - 2-3

ER -