Spreading of streptomycin antibiotic resistance gene in Escherichia coli plasmids demonstrated by Southern blot analysis

A. Hajnal, A. Falus

Research output: Contribution to journalArticle

Abstract

Plasmids from E. coli strains of 38 donors were transconjugated to common recipient SY663 Escherichia coli K12. The restriction patterns of the isolated plasmids were highly heterogenous. However, the streptomycin (Sm) resistance genes of the plasmids were identical or closely homologous in 29 of the 33 plasmids conferring Sm resistance. These data were based on Southern blot analysis, using the Sm resistance gene (encoding aminoglycoside phosphoryl transferase) as probe cut out from pBP1 plasmid. Our data suggest an extensive spreading of streptomycin resistance gene of this type.

Original languageEnglish
Pages (from-to)441-447
Number of pages7
JournalMicrobiology and Immunology
Volume33
Issue number6
Publication statusPublished - 1989

Fingerprint

Streptomycin
Microbial Drug Resistance
Southern Blotting
Plasmids
Escherichia coli
Genes
Escherichia coli K12
Aminoglycosides
Transferases

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Microbiology
  • Microbiology (medical)

Cite this

@article{0552d5902eab440fb916d95c220fc146,
title = "Spreading of streptomycin antibiotic resistance gene in Escherichia coli plasmids demonstrated by Southern blot analysis",
abstract = "Plasmids from E. coli strains of 38 donors were transconjugated to common recipient SY663 Escherichia coli K12. The restriction patterns of the isolated plasmids were highly heterogenous. However, the streptomycin (Sm) resistance genes of the plasmids were identical or closely homologous in 29 of the 33 plasmids conferring Sm resistance. These data were based on Southern blot analysis, using the Sm resistance gene (encoding aminoglycoside phosphoryl transferase) as probe cut out from pBP1 plasmid. Our data suggest an extensive spreading of streptomycin resistance gene of this type.",
author = "A. Hajnal and A. Falus",
year = "1989",
language = "English",
volume = "33",
pages = "441--447",
journal = "Microbiology and Immunology",
issn = "0385-5600",
publisher = "Center for Academic Publications Japan",
number = "6",

}

TY - JOUR

T1 - Spreading of streptomycin antibiotic resistance gene in Escherichia coli plasmids demonstrated by Southern blot analysis

AU - Hajnal, A.

AU - Falus, A.

PY - 1989

Y1 - 1989

N2 - Plasmids from E. coli strains of 38 donors were transconjugated to common recipient SY663 Escherichia coli K12. The restriction patterns of the isolated plasmids were highly heterogenous. However, the streptomycin (Sm) resistance genes of the plasmids were identical or closely homologous in 29 of the 33 plasmids conferring Sm resistance. These data were based on Southern blot analysis, using the Sm resistance gene (encoding aminoglycoside phosphoryl transferase) as probe cut out from pBP1 plasmid. Our data suggest an extensive spreading of streptomycin resistance gene of this type.

AB - Plasmids from E. coli strains of 38 donors were transconjugated to common recipient SY663 Escherichia coli K12. The restriction patterns of the isolated plasmids were highly heterogenous. However, the streptomycin (Sm) resistance genes of the plasmids were identical or closely homologous in 29 of the 33 plasmids conferring Sm resistance. These data were based on Southern blot analysis, using the Sm resistance gene (encoding aminoglycoside phosphoryl transferase) as probe cut out from pBP1 plasmid. Our data suggest an extensive spreading of streptomycin resistance gene of this type.

UR - http://www.scopus.com/inward/record.url?scp=0024341868&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024341868&partnerID=8YFLogxK

M3 - Article

C2 - 2671608

AN - SCOPUS:0024341868

VL - 33

SP - 441

EP - 447

JO - Microbiology and Immunology

JF - Microbiology and Immunology

SN - 0385-5600

IS - 6

ER -