Spreading of Streptomycin Antibiotic Resistance Gene in Escherichia coli Plasmids Demonstrated by Southern Blot Analysis

Anna Hajnal, Andras Falus

Research output: Contribution to journalArticle

Abstract

Plasmids from E. coli strains of 38 donors were transconjugated to common recipient SY663 Escherichia coli K12. The restriction patterns of the isolated plasmids were highly heterogenous. However, the streptomycin (Sm) resistance genes of the plasmids were identical or closely homologous in 29 of the 33 plasmids conferring Sm resistance. These data were based on Southern blot analysis, using the Sm resistance gene (encoding aminoglycoside phosphoryl transferase) as probe cut out from pBPl plasmid. Our data suggest an extensive spreading of streptomycin resistance gene of this type.

Original languageEnglish
Pages (from-to)1095-1103
Number of pages9
JournalMicrobiology and Immunology
Volume33
Issue number6
Publication statusPublished - Jan 1 1989

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

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