Spotted-dick, a zinc-finger protein of Drosophila required for expression of Orc4 and S phase

Andrew R. Page, Andras Kovacs, P. Deák, Tibor Torok, I. Kiss, Paulo Dario, Cristina Bastos, Pedro Batista, Rui Gomes, Hiro Ohkura, Steven Russell, David M. Glover

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The highly condensed chromosomes and chromosome breaks in mitotic cells of a Drosophila mutant, spotted-dick/pita, are the consequence of defects in DNA replication. Reduction of levels of Spotted-dick protein, by either RNAi or mutation, leads to the accumulation of cells that have DNA content intermediate to 2N and 4N in proliferating tissues and also compromises endoreduplication in larval salivary glands. The Spotted-dick Zinc-finger protein is present in the nuclei of cells committed to proliferation but necessary in cells undertaking S phase. We show that Spotted-dick/Pita functions as a transcription factor and that, in cultured S2 cells, it is an activator of expression of some 30 genes that include the Orc4 gene, required for initiation of DNA replication. Chromatin immunoprecipitation indicates that it associates with the genes that it activates in S2 cells together with other sites that could represent genes activated in other tissues. We discuss the role of Spotted-dick in the coordination of cellular growth and DNA replication.

Original languageEnglish
Pages (from-to)4304-4315
Number of pages12
JournalEMBO Journal
Volume24
Issue number24
DOIs
Publication statusPublished - Dec 21 2005

Fingerprint

Drosophila Proteins
Zinc Fingers
S Phase
Zinc
Genes
DNA Replication
DNA
Chromosomes
Endoreduplication
Tissue
Chromosome Breakage
Chromatin Immunoprecipitation
RNA Interference
Salivary Glands
Cell Nucleus
Drosophila
Chromatin
Cultured Cells
Proteins
Transcription Factors

Keywords

  • Chromatin immunoprecipitation
  • Endoreduplication
  • Microarray
  • Mitotis
  • S phase

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Spotted-dick, a zinc-finger protein of Drosophila required for expression of Orc4 and S phase. / Page, Andrew R.; Kovacs, Andras; Deák, P.; Torok, Tibor; Kiss, I.; Dario, Paulo; Bastos, Cristina; Batista, Pedro; Gomes, Rui; Ohkura, Hiro; Russell, Steven; Glover, David M.

In: EMBO Journal, Vol. 24, No. 24, 21.12.2005, p. 4304-4315.

Research output: Contribution to journalArticle

Page, AR, Kovacs, A, Deák, P, Torok, T, Kiss, I, Dario, P, Bastos, C, Batista, P, Gomes, R, Ohkura, H, Russell, S & Glover, DM 2005, 'Spotted-dick, a zinc-finger protein of Drosophila required for expression of Orc4 and S phase', EMBO Journal, vol. 24, no. 24, pp. 4304-4315. https://doi.org/10.1038/sj.emboj.7600890
Page, Andrew R. ; Kovacs, Andras ; Deák, P. ; Torok, Tibor ; Kiss, I. ; Dario, Paulo ; Bastos, Cristina ; Batista, Pedro ; Gomes, Rui ; Ohkura, Hiro ; Russell, Steven ; Glover, David M. / Spotted-dick, a zinc-finger protein of Drosophila required for expression of Orc4 and S phase. In: EMBO Journal. 2005 ; Vol. 24, No. 24. pp. 4304-4315.
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