Spotlight on a new heme oxygenase pathway: Testosterone-induced shifts in cardiac oxidant/antioxidant status

Renáta Szabó, Denise Börzsei, Krisztina Kupai, Alexandra Hoffmann, R. Gesztelyi, A. Berkó, C. Varga, A. Pósa

Research output: Contribution to journalArticle

Abstract

A low testosterone level contributes to the development of oxidative damages; however, the cardiovascular effects of exogenous hormone therapy are not well elucidated. The aim of our work is to study the association of the testosterone level, antioxidant/oxidant system, and anti-inflammatory status related to the heme oxygenase (HO) system. To determine the effects of testosterone, 10-week-old, and 24-month-old sham-operated and castrated male Wistar rats were used. One part of the castrated animals was daily treated with 2.5 mg/kg cyproterone acetate, while the hormone replacement therapy was performed via an i.m. injection of a dose of 8.0 mg testosterone undecanoate/kg/once a week. The plasma testosterone level, the activity of HO and myeloperoxidase (MPO) enzymes; the concentrations of the HO-1, tumor necrosis alpha (TNF-α), and cyclic guanosine monophosphate (cGMP), as well as the total level of glutathione (GSH + GSSG) were determined from the cardiac left ventricle. In accordance with the testosterone values, the aging process and castration resulted in a decrease in antioxidant HO activity, HO-1 and cGMP concentrations and in the level of GSH + GSSG, whereas the inflammatory TNF-α and MPO activity significantly increased. Testosterone therapy was able to restore the physiological values. Our results clearly show that testosterone replacement therapy increases the antioxidant status and mitigates the inflammatory parameters via the modulation of the HO system.

Original languageEnglish
Article number288
JournalAntioxidants
Volume8
Issue number8
DOIs
Publication statusPublished - Aug 1 2019

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Heme Oxygenase (Decyclizing)
Oxidants
Testosterone
Antioxidants
Heme Oxygenase-1
Glutathione Disulfide
Cyclic GMP
Peroxidase
Heart Ventricles
Hormones
Cyproterone Acetate
Castration
Hormone Replacement Therapy
Glutathione
Wistar Rats
Rats
Tumors
Animals
Anti-Inflammatory Agents
Necrosis

Keywords

  • Antioxidant
  • Heme oxygenase
  • Inflammation
  • Testosterone

ASJC Scopus subject areas

  • Physiology
  • Biochemistry
  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Spotlight on a new heme oxygenase pathway : Testosterone-induced shifts in cardiac oxidant/antioxidant status. / Szabó, Renáta; Börzsei, Denise; Kupai, Krisztina; Hoffmann, Alexandra; Gesztelyi, R.; Berkó, A.; Varga, C.; Pósa, A.

In: Antioxidants, Vol. 8, No. 8, 288, 01.08.2019.

Research output: Contribution to journalArticle

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