Spot and mobile-phase front anomalies in planar (thin-layer) chromatography

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The advantages and variations of thin-layer chromatography are described in detail. The displacement mode of development is generated in a relatively short distance, and the fully developed displacement train concentrates sample components even from an elongated spot. Two-dimensional TLC using both elution and displacement thin-layer chromatography enables excellent separation of spots; results from 2D TLC cannot, however, be derived directly from two one-dimensional runs. Secondary mobile-phase fronts occur because of the dry state of stationary phase, which absorbs the mobile phase. Occasional problems with the reproducibility of RF values arise because of adsorption of the mobile phase components by the dry stationary phase and evaporation at the solvent front. The use of 2D TLC in pharmacognosy enables separation of the components and also helps in the planning of preparative-scale separations by classical column chromatography and/or HPLC.

Original languageEnglish
Pages (from-to)S57-S62
Issue numberSUPPL. 13
Publication statusPublished - Oct 1 2005



  • Elution-displacement TLC
  • Elution-elution TLC
  • Front of total wetness
  • Secondary fronts
  • Thin-layer chromatography

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

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