Spontaneous ultraweak light emission from respiring spinach leaf mitochondria

É. Hideg, Masaki Kobayashi, Humio Inaba

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Mitochondria isolated from spinach leaves showed spontaneous ultraweak light emission depending on the respiration with succinate. This emission was sensitive to respiratory inhibitors, such as, antimycin-A and potassium cyanide. Mitochondrial photoemission was inhibited by azide and salicylhyrdoxamic acid and enhanced in the presence of 1,4-diazabicyclo[2,2,2]-octane (DABCO), implying a role of singlet oxygen in the process. Ultraweak light emission and singlet oxygen production, the latter determined spectroscopically in experiments parallel to light measurements, exhibited similar changes upon various chemical treatments. The emission spectrum of ultraweak light closely resembles that of chemiluminescence from an in vitro (linoleic acid + peroxidase) lipid peroxidation. We suggest that the observed phenomenon is a result of the peroxidation of mitochondrial membrane lipids initiated by the activity of the respiratory electron transport, probably at the cytochrome c site.

Original languageEnglish
Pages (from-to)27-31
Number of pages5
JournalBBA - Bioenergetics
Volume1098
Issue number1
DOIs
Publication statusPublished - Dec 3 1991

Fingerprint

Singlet Oxygen
Mitochondria
Spinacia oleracea
Spontaneous emission
Light emission
Light measurement
Potassium Cyanide
Antimycin A
Light
Chemiluminescence
Azides
Photoemission
Succinic Acid
Linoleic Acid
Membrane Lipids
Cytochromes c
Peroxidase
Respiratory Transport
Lipids
Acids

Keywords

  • Emission spectrum
  • Lipid peroxidation
  • Mitochondrion
  • Spinach
  • Ultraweak light emission

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Molecular Biology
  • Biochemistry

Cite this

Spontaneous ultraweak light emission from respiring spinach leaf mitochondria. / Hideg, É.; Kobayashi, Masaki; Inaba, Humio.

In: BBA - Bioenergetics, Vol. 1098, No. 1, 03.12.1991, p. 27-31.

Research output: Contribution to journalArticle

Hideg, É. ; Kobayashi, Masaki ; Inaba, Humio. / Spontaneous ultraweak light emission from respiring spinach leaf mitochondria. In: BBA - Bioenergetics. 1991 ; Vol. 1098, No. 1. pp. 27-31.
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N2 - Mitochondria isolated from spinach leaves showed spontaneous ultraweak light emission depending on the respiration with succinate. This emission was sensitive to respiratory inhibitors, such as, antimycin-A and potassium cyanide. Mitochondrial photoemission was inhibited by azide and salicylhyrdoxamic acid and enhanced in the presence of 1,4-diazabicyclo[2,2,2]-octane (DABCO), implying a role of singlet oxygen in the process. Ultraweak light emission and singlet oxygen production, the latter determined spectroscopically in experiments parallel to light measurements, exhibited similar changes upon various chemical treatments. The emission spectrum of ultraweak light closely resembles that of chemiluminescence from an in vitro (linoleic acid + peroxidase) lipid peroxidation. We suggest that the observed phenomenon is a result of the peroxidation of mitochondrial membrane lipids initiated by the activity of the respiratory electron transport, probably at the cytochrome c site.

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