Spontaneous methylation of dipalmitoyl phosphatidyl-ethanolamine by formaldehyde

M. Szogyi, T. Cserhati

Research output: Contribution to journalArticle


The time dependence of the interaction between dipalmitoyl-phosphatidylethanolamine (DPPE) and formaldehyde was determined at various pH values and temperatures by differential scanning calorimetry. The reaction products were separated with reversed-phase thin-layer chromatography. Formaldehyde decreased the main transition temperature and the enthalpy of DPPE and increased the peak half width. The interaction depended significantly on the grade of dissociation of the amino group of DPPE, it involves hydrogen bonding and chemical reactions. The chromatographic separations make probable that the methylation occurs consecutively from N-methyl-ethanolamine to N,N-dimethyl-ethanolamine.

Original languageEnglish
Pages (from-to)545-554
Number of pages10
JournalBiochemistry International
Issue number3
Publication statusPublished - Aug 23 1990


ASJC Scopus subject areas

  • Biochemistry

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