Spontaneous insertion of a B2 element in the Ptpn6 gene drives a systemic autoinflammatory disease in mice resembling neutrophilic dermatosis in humans

Andrew B. Nesterovitch, Sandor Szanto, Andrea Gonda, Tamas Bardos, Katalin Kis-Toth, Vyacheslav A. Adarichev, Katalin Olasz, Sheida Ghassemi-Najad, Mark D. Hoffman, Michael D. Tharp, Katalin Mikecz, Tibor T. Glant

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23 Citations (Scopus)

Abstract

We found a spontaneous autosomal mutation in a mouse leading to neutrophil infiltration with ulceration in the upper dermis of homozygous offspring. These animals had increased neutrophil numbers, associated with normal lymphocyte count, in peripheral blood and bone marrow, suggesting a myeloproliferative disorder; however, granulocyte precursor proliferation in bone marrow was actually reduced (because circulating neutrophils were less susceptible to apoptosis). Neutrophil infiltration of the skin and other organs and high serum levels of immunoglobulins and autoantibodies, cytokines, and acute-phase proteins were additional abnormalities, all of which could be reduced by high-dose corticosteroid treatment or neutrophil depletion by antibodies. Use of genome-wide screening localized the mutation within an 0.4-Mbp region on mouse chromosome 6. We identified insertion of a B2 element in exon 6 of the Ptpn6 gene (protein tyrosine phosphatase, non-receptor type 6; also known as Shp-1). This insertion involves amino acid substitutions that significantly reduced the enzyme activity in mice homozygous for the mutation. Disease onset was delayed, and the clinical phenotype was milder than the phenotypes of other Ptpn6-mutants described in motheaten (me, mev) mice; we designated this new genotype as Ptpn6meB2/meB2 and the phenotype as meB2. This new phenotype encompasses an autoinflammatory disease showing similarities to many aspects of the so-called neutrophilic dermatoses, a heterogeneous group of skin diseases with unknown etiology in humans.

Original languageEnglish
Pages (from-to)1701-1714
Number of pages14
JournalAmerican Journal of Pathology
Volume178
Issue number4
DOIs
Publication statusPublished - Apr 2011

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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    Nesterovitch, A. B., Szanto, S., Gonda, A., Bardos, T., Kis-Toth, K., Adarichev, V. A., Olasz, K., Ghassemi-Najad, S., Hoffman, M. D., Tharp, M. D., Mikecz, K., & Glant, T. T. (2011). Spontaneous insertion of a B2 element in the Ptpn6 gene drives a systemic autoinflammatory disease in mice resembling neutrophilic dermatosis in humans. American Journal of Pathology, 178(4), 1701-1714. https://doi.org/10.1016/j.ajpath.2010.12.053