Spontaneous and induced differentiation of human melanoma cells

I. Valyi-Nagy, I. M. Shih, T. Gyorfi, D. Greenstein, I. Juhász, D. E. Elder, M. Herlyn

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Malignant melanoma cells can differentiate spontaneously in vivo and in vitro into cells with a finite lifespan. Analysis of differentiating cells from primary melanomas in culture revealed a flat, fibroblast-like morphology and expression of the fibroblast-associated marker leucine aminopeptidase (LAP). Differentiation was also observed in a minor sub-population of permanent cell lines derived fromo metastatic lesions. An experimental model of melanoma cell differentiation was then developed, using the pyrimidine analog bromodeoxyuridine (BUdR). BUdR-treated cells had a flat morphology, were contact-inhibited, had up to 20-fold increased surface area, expressed LAP, no longer proliferated anchorage-independently in soft agar, and 3 out of 4 cell lines were non-tumorigenic in athymic nude mice. Our results show that models of differentiation of melanoma cells can be established that help to define pathways of differentiation.

Original languageEnglish
Pages (from-to)159-165
Number of pages7
JournalInternational Journal of Cancer
Volume54
Issue number1
Publication statusPublished - 1993

Fingerprint

Melanoma
Bromodeoxyuridine
Leucyl Aminopeptidase
Nude Mice
Cell Differentiation
Fibroblasts
Cell Line
Experimental Melanomas
Agar
Theoretical Models
Population

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Valyi-Nagy, I., Shih, I. M., Gyorfi, T., Greenstein, D., Juhász, I., Elder, D. E., & Herlyn, M. (1993). Spontaneous and induced differentiation of human melanoma cells. International Journal of Cancer, 54(1), 159-165.

Spontaneous and induced differentiation of human melanoma cells. / Valyi-Nagy, I.; Shih, I. M.; Gyorfi, T.; Greenstein, D.; Juhász, I.; Elder, D. E.; Herlyn, M.

In: International Journal of Cancer, Vol. 54, No. 1, 1993, p. 159-165.

Research output: Contribution to journalArticle

Valyi-Nagy, I, Shih, IM, Gyorfi, T, Greenstein, D, Juhász, I, Elder, DE & Herlyn, M 1993, 'Spontaneous and induced differentiation of human melanoma cells', International Journal of Cancer, vol. 54, no. 1, pp. 159-165.
Valyi-Nagy I, Shih IM, Gyorfi T, Greenstein D, Juhász I, Elder DE et al. Spontaneous and induced differentiation of human melanoma cells. International Journal of Cancer. 1993;54(1):159-165.
Valyi-Nagy, I. ; Shih, I. M. ; Gyorfi, T. ; Greenstein, D. ; Juhász, I. ; Elder, D. E. ; Herlyn, M. / Spontaneous and induced differentiation of human melanoma cells. In: International Journal of Cancer. 1993 ; Vol. 54, No. 1. pp. 159-165.
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