Spin label EPR study of lipid solvation of supramolecular photosynthetic protein complexes in thylakoids

Anabella Ivancich, LászlóI Horváth, Magdolna Droppa, G. Horváth, T. Farkas

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Lipid-protein association in the chloroplast membrane and its various thylakoid fractions from higher plants, namely pea and maize, rich in Photosystem I (PSI) and Photosystem II (PSII), respectively, were studied using EPR spectroscopy of spin-labelled lipid molecules. All the EPR spectra consisted of two spectral components corresponding to bulk fluid lipids and solvation lipids motionally restricted at the hydrophobic surface of membrane proteins. Spin-labelled stearic acid and phosphatidylglycerol exhibited marked selectivity towards the supramolecular protein complexes of both PSI and PSII although to different extent. In addition, lipid-protein titration experiments are described for partially delipidated PSII-enriched membrane fractions of pea chloroplasts, incorporating unlabelled egg phosphatidylcholine prior to or after the incorporation of spin-labelled lipids. Two sets of solvation sites were resolved by timed labelling experiments and a significant result of these studies was that a well-defined population of solvation sites (approx. 100 mol lipids/820 kDa protein) was rapidly exchanged by laterally diffusing membrane lipids, while other solvation sites (approx. 50 mol lipids/820 kDa protein) were exchanged much slower or not exchanged at all.

Original languageEnglish
Pages (from-to)51-56
Number of pages6
JournalBBA - Biomembranes
Volume1196
Issue number1
DOIs
Publication statusPublished - Nov 23 1994

Fingerprint

Photosynthetic Reaction Center Complex Proteins
Spin Labels
Thylakoids
Solvation
Paramagnetic resonance
Lipids
Photosystem II Protein Complex
Proteins
Photosystem I Protein Complex
Peas
Chloroplasts
Membrane Proteins
Membranes
Phosphatidylglycerols
Membrane Lipids
Phosphatidylcholines
Titration
Labeling
Zea mays
Ovum

Keywords

  • EPR
  • Lipid-protein interaction
  • Thylakoid

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology

Cite this

Spin label EPR study of lipid solvation of supramolecular photosynthetic protein complexes in thylakoids. / Ivancich, Anabella; Horváth, LászlóI; Droppa, Magdolna; Horváth, G.; Farkas, T.

In: BBA - Biomembranes, Vol. 1196, No. 1, 23.11.1994, p. 51-56.

Research output: Contribution to journalArticle

Ivancich, Anabella ; Horváth, LászlóI ; Droppa, Magdolna ; Horváth, G. ; Farkas, T. / Spin label EPR study of lipid solvation of supramolecular photosynthetic protein complexes in thylakoids. In: BBA - Biomembranes. 1994 ; Vol. 1196, No. 1. pp. 51-56.
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