Spiegelmers as potential receptors for cTnI diagnostics

Zsuzsanna Szeitner, Anna Doleschall, Marina Varga, Katalin Keltai, Katalin Révész, Róbert E. Gyurcsányi, Tamás Mészáros

Research output: Contribution to journalArticle

Abstract

We demonstrate the first application of a nuclease resistant aptamer, the so-called Spiegelmer, in a sandwich-type affinity assay by quantitative assessment of cardiac Troponin I (cTnI) in blood serum samples. To this end, we used a bead-based homogenous proximity assay (AlphaLISA) in which luminescence is generated by the Spiegelmer and antibody-modified donor and acceptor beads brought into proximity by their binding to cTnI.

Original languageEnglish
Pages (from-to)5091-5093
Number of pages3
JournalAnalytical Methods
Volume9
Issue number35
DOIs
Publication statusPublished - Sep 21 2017

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ASJC Scopus subject areas

  • Analytical Chemistry
  • Chemical Engineering(all)
  • Engineering(all)

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