Sperm cryopreservation of aquatic species

Research output: Chapter in Book/Report/Conference proceedingChapter


Cryopreservation of fish sperm is feasible, and methods have been developed for several hundred species. The procedure includes the collection of sperm and assessment of its quality, dilution in suitable extenders, addition of cryoprotectants, loading into freezing devices, cooling, storage, thawing, and finally post-thaw use of sperm. An alternative to traditional freezing methods is the vitrification of sperm which is promising primarily in smaller model fish species. A wide variety of protocols are available in the literature; however, in spite of the significant progress made by the scientific community, commercial application of fish sperm cryopreservation is still very limited.

Original languageEnglish
Title of host publicationReproduction in Aquatic Animals
Subtitle of host publicationFrom Basic Biology to Aquaculture Technology
PublisherSpringer Singapore
Number of pages14
ISBN (Electronic)9789811522901
ISBN (Print)9789811522895
Publication statusPublished - Jan 1 2020


  • Cryopreservation
  • Freezing
  • Quality
  • Sperm
  • Vitrification

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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  • Cite this

    Horváth, A., & Urbányi, B. (2020). Sperm cryopreservation of aquatic species. In Reproduction in Aquatic Animals: From Basic Biology to Aquaculture Technology (pp. 321-334). Springer Singapore. https://doi.org/10.1007/978-981-15-2290-1_16