Cryopreservation of fish sperm is feasible, and methods have been developed for several hundred species. The procedure includes the collection of sperm and assessment of its quality, dilution in suitable extenders, addition of cryoprotectants, loading into freezing devices, cooling, storage, thawing, and finally post-thaw use of sperm. An alternative to traditional freezing methods is the vitrification of sperm which is promising primarily in smaller model fish species. A wide variety of protocols are available in the literature; however, in spite of the significant progress made by the scientific community, commercial application of fish sperm cryopreservation is still very limited.
|Title of host publication||Reproduction in Aquatic Animals|
|Subtitle of host publication||From Basic Biology to Aquaculture Technology|
|Number of pages||14|
|Publication status||Published - Jan 1 2020|
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Agricultural and Biological Sciences(all)