Sperm chromatin structure and sperm morphology: Their association with fertility in AI-dairy Ayrshire sires

S. Nagy, A. Johannisson, T. Wahlsten, R. Ijäs, M. Andersson, H. Rodriguez-Martinez

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Prognostic relations between sperm variables and sire fertility are yet elusive. A retrospective analysis of sperm morphology and chromatin stability (studied using sperm chromatin structure assay [SCSA]) and their relation to fertility after AI (as proportions of 60 days of nonreturn to estrous [NRR], corrected NRR, or calving rate) was studied with preselected frozen semen doses from a group (N = 43) of AI-sires of the Finnish Ayrshire breed composed of 50% subfertile bulls (55% NRR). Fertility, indicated by all three parameters, correlated significantly only with the percentage of morphologically normal spermatozoa, a variable which negatively correlated with the percentage of DNA fragmentation at the time of SCSA, thus confirming the value of always having high numbers of morphologically normal spermatozoa in AI-doses. Proportions of major sperm defects also related to fertility but only when considering corrected NRR, not with calving rate, indicating that proportions of normal spermatozoa, a value surpassing differences between sperm laboratory screening methods, might be valuable and could be easily made routine by the industry. Though SCSA as a method is being contested for DNA- and chromatin analyses in the light of epigenetic changes, a particular parameter, the High Green fluorescence, showed the highest values for sperm doses collected from bulls having meiotic problems and containing a high proportion of diploid spermatozoa (approximately 20%) and also in bulls having a reciprocal chromosomal translocation, thus suggesting such a parameter might be useable to discriminate which bulls ought to be studied in more detail, including cytogenetic analyses.

Original languageEnglish
Pages (from-to)1153-1161
Number of pages9
JournalTheriogenology
Volume79
Issue number8
DOIs
Publication statusPublished - May 2013

Fingerprint

Ayrshire (cattle breed)
sires
Chromatin
Fertility
chromatin
Spermatozoa
dairies
spermatozoa
bulls
calving rate
assays
dosage
Semen Preservation
Genetic Translocation
Cytogenetic Analysis
DNA fragmentation
DNA Fragmentation
Diploidy
cytogenetics
Epigenomics

Keywords

  • Bull
  • Fertility
  • Sperm chromatin integrity
  • Sperm morphology

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Equine
  • Food Animals
  • Small Animals

Cite this

Sperm chromatin structure and sperm morphology : Their association with fertility in AI-dairy Ayrshire sires. / Nagy, S.; Johannisson, A.; Wahlsten, T.; Ijäs, R.; Andersson, M.; Rodriguez-Martinez, H.

In: Theriogenology, Vol. 79, No. 8, 05.2013, p. 1153-1161.

Research output: Contribution to journalArticle

Nagy, S, Johannisson, A, Wahlsten, T, Ijäs, R, Andersson, M & Rodriguez-Martinez, H 2013, 'Sperm chromatin structure and sperm morphology: Their association with fertility in AI-dairy Ayrshire sires', Theriogenology, vol. 79, no. 8, pp. 1153-1161. https://doi.org/10.1016/j.theriogenology.2013.02.011
Nagy, S. ; Johannisson, A. ; Wahlsten, T. ; Ijäs, R. ; Andersson, M. ; Rodriguez-Martinez, H. / Sperm chromatin structure and sperm morphology : Their association with fertility in AI-dairy Ayrshire sires. In: Theriogenology. 2013 ; Vol. 79, No. 8. pp. 1153-1161.
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