Spectroscopy and modelling of the cytoplasmic domain of the gamma-subunit of the high affinity immunoglobulin E receptor.

G. J. Anderson, R. R. Biekofsky, M. Zloh, G. Tóth, I. Toth, E. Benedetti, W. A. Gibbons

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The high affinity receptor for IgE, Fc epsilon RI, is responsible for immediate hypersensitivity reactions. In rodents Fc epsilon RI is a tetrameric complex, alpha beta gamma 2 of non-covalently attached subunits: one IgE-binding alpha subunit with the binding site in the extracellular part of the chain, one beta-subunit and a dimer of disulphide linked gamma-subunits. Although there is an increasing evidence that the gamma-subunit chains are important signalling proteins that appear to function through a common Tyr-Leu-Tyr-Leu amino acid motif present in their cytoplasmic tails, which link the ligand binding specificity of their associated chains to signal transduction pathways, many questions related to conformation and function of this subunit remain to be answered. In the present work, the 36-residue cytoplasmic domain of the gamma-subunit has been synthesized and conformational studies by the combined use of Fourier transform infrared (FTIR), circular dichroism (CD) and nuclear magnetic resonance (NMR) have been performed. Based on the constraints found by these methods, conformational models of the cytoplasmic tail of the gamma-subunit are proposed and discussed.

Original languageEnglish
Pages (from-to)31-38
Number of pages8
JournalBiomedical peptides, proteins & nucleic acids : structure, synthesis & biological activity
Volume1
Issue number1
Publication statusPublished - 1994

Fingerprint

IgE Receptors
Spectrum Analysis
Tail
Immediate Hypersensitivity
Amino Acid Motifs
Fourier Analysis
Circular Dichroism
Disulfides
Immunoglobulin E
Rodentia
Signal Transduction
Magnetic Resonance Spectroscopy
Binding Sites
Ligands
Proteins

Cite this

Spectroscopy and modelling of the cytoplasmic domain of the gamma-subunit of the high affinity immunoglobulin E receptor. / Anderson, G. J.; Biekofsky, R. R.; Zloh, M.; Tóth, G.; Toth, I.; Benedetti, E.; Gibbons, W. A.

In: Biomedical peptides, proteins & nucleic acids : structure, synthesis & biological activity, Vol. 1, No. 1, 1994, p. 31-38.

Research output: Contribution to journalArticle

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AU - Biekofsky, R. R.

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AU - Tóth, G.

AU - Toth, I.

AU - Benedetti, E.

AU - Gibbons, W. A.

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