Spectroscopic properties of protochlorophyll forms in Triton X-100 detergent micelles

B. Böddi, Katalin Kovács, F. Láng

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Abstract

Protochlorophyll (PChl) forms were performed in Triton X-100 detergent micelles. The concentration of Triton X-100 was 7·10-4 M (above the critical micellar concentration); the concentration of PChl varied between 1.6·10-5 and 1.8·10-4 M. Absorption, fluorescence and circular dichroism (CD) spectra were registered. The absorption spectra were resolved into Gaussian components by computer analysis. PChl forms with absorption bands at 632-634, 638, 652-654, 663-664, 668 and 676 nm and with fluorescence emission bands at 634-636, 640-644, 652-655, 677-678, 686 and 694-696 nm were observed in micellar solutions of different PChl concentrations. The CD spectra showed a strong dependence on the concentration of PChl: positive CD signals or positive Cotton effects were observed in the vicinity of 650 nm. The intensity of these signals increased in parallel with increasing concentration of PChl. No CD signals were found in the region of the longer wavelength absorption bands. These data show that the PChl exists in many different forms in this system, and the spectroscopic properties of these forms are determined by different molecular interactions viz., interactions of PChl with Triton X-100 or water molecules and/or by the aggregation of PChl.

Original languageEnglish
Pages (from-to)320-326
Number of pages7
JournalBBA - Bioenergetics
Volume722
Issue number2
DOIs
Publication statusPublished - Feb 17 1983

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Keywords

  • Detergent micelle
  • Protochlorophyll
  • Spectroscopy
  • Triton X-100

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

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