Spectroscopic and OPLC identification and measurement of formaldehyde and potential formaldehyde generators in macroscopic fungi

G. Kátay, Maricella Adrian-Romero, Z. Németh, Gerald Blunden, E. Tyihák, Levente Albert

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Optimum-performance laminar chromatography (previously known as overpressured-layer chromatography; OPLC) exploits the advantages of the optimum laminar flow of mobile phase obtained by use of pump to introduce the mobile phase to the adsorbent layer in an automated, microprocessor-controlled separation system. The optimized flow profile in OPLC is the basis of the efficiency of this new technique. The attractiveness of OPLC is particularly apparent from the width of the separation surface (large number of samples). OPLC has enabled improvement of the velocity profile with a decrease in eddy diffusion. OPLC can be used for efficient separation of formaldehyde (HCHO) and some betaines, potential HCHO generators, in macroscopic fungi. Endogenous HCHO is determined, after conversion to formaldemethone, and characterized from proton magnetic resonance spectroscopic (1H NMR) and electron-impact mass spectrometric (EIMS) data. The results show that macroscopic fungi contain moderate levels of HCHO compared with, for example, the leaves of certain higher plant species. Among the betaines, l-carnitine and glycinebetaine were identified by matrix-assisted laser-desorption ionization mass spectrometry (MALDI MS), 1H NMR spectroscopy, and OPLC. Data prove that automated OPLC, a new separation technique, is suitable for efficient separation of natural substances from a large number of samples in one separation; it is, therefore, a prospective complementary methodological direction among separation techniques.

Original languageEnglish
Pages (from-to)28-33
Number of pages6
JournalJournal of Planar Chromatography - Modern TLC
Volume15
Issue number1
Publication statusPublished - 2002

Fingerprint

Betaine
Fungi
Formaldehyde
Chromatography
Magnetic Resonance Spectroscopy
Carnitine
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Microcomputers
Protons
Electrons
Nuclear magnetic resonance
Proton Magnetic Resonance Spectroscopy
Laminar flow
Adsorbents
Nuclear magnetic resonance spectroscopy
Ionization
Mass spectrometry
Microprocessor chips
Desorption
Pumps

Keywords

  • Betaines
  • Formaldehyde
  • Macroscopic fungi
  • Optimum performance laminar/overpressured layer chromatography (OPLC)
  • Spectroscopy

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Spectroscopic and OPLC identification and measurement of formaldehyde and potential formaldehyde generators in macroscopic fungi. / Kátay, G.; Adrian-Romero, Maricella; Németh, Z.; Blunden, Gerald; Tyihák, E.; Albert, Levente.

In: Journal of Planar Chromatography - Modern TLC, Vol. 15, No. 1, 2002, p. 28-33.

Research output: Contribution to journalArticle

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