Spectrophotometric determination of lysine in the presence of other amino acids and proteins using furfuraldehyde

Ibolya Molnár-Perl, Margit Pintér-Szakás

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The selective reaction of lysine, δ-hydroxylysine, ornithine and α,ε-diaminopimelic acid with furfuraldehyde, in anhydrous media, was investigated at 80, 85, 90 and 95°C by spectrophometry, in the presence of various additional materials, such as potassium pyrosulphite, ascorbic acid and lead(II) acetate. Second-order rate constants and molar absorptivities were determined. Optimum conditions were established for the selective determination of lysine in the presence of intact proteins or in protein hydrolysates in the presence of a wide range of other amino acids including ornithine. Under optimum conditions, in the concentration range 2 × 10-8-3 × 10-7 M, molar absorptivities and reproducibility data were measured with a standard error of 0.9-5.0%, depending on the procedure applied and on the amount of lysine present.

Original languageEnglish
Pages (from-to)209-216
Number of pages8
JournalAnalytica Chimica Acta
Volume257
Issue number2
DOIs
Publication statusPublished - Feb 25 1992

Keywords

  • Amino acids
  • Lysine
  • Proteins
  • Spectrophotometry

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

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