Spectrokinetic characterization of photoactive yellow protein films for integrated optical applications

Szilvia Krekic, Dávid Nagy, Stefka G. Taneva, László Fábián, L. Zimányi, A. Dér

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

In this paper, the photocycle of the dried photoactive yellow protein film has been investigated in different humidity environments, in order to characterize its nonlinear optical properties for possible integrated optical applications. The light-induced spectral changes of the protein films were monitored by an optical multichannel analyser set-up, while the accompanying refractive index changes were measured with the optical waveguide lightmode spectroscopy method. To determine the number and kinetics of spectral intermediates in the photocycle, the absorption kinetic data were analysed by singular value decomposition and multiexponential fitting methods, whose results were used in a subsequent step of fitting a photocycle model to the data. The absorption signals of the films were found to be in strong correlation with the measured light-induced refractive index changes, whose size and kinetics imply that photoactive yellow protein may be a good alternative for utilization as an active nonlinear optical material in future integrated optical applications.

Original languageEnglish
JournalEuropean Biophysics Journal
DOIs
Publication statusPublished - Jan 1 2019

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Refractometry
Light
Proteins
Humidity
Spectrum Analysis

Keywords

  • Biophotonics
  • Integrated optics
  • Kinetic absorption spectroscopy
  • Optical waveguide lightmode spectroscopy
  • Photoactive yellow protein

ASJC Scopus subject areas

  • Biophysics

Cite this

Spectrokinetic characterization of photoactive yellow protein films for integrated optical applications. / Krekic, Szilvia; Nagy, Dávid; Taneva, Stefka G.; Fábián, László; Zimányi, L.; Dér, A.

In: European Biophysics Journal, 01.01.2019.

Research output: Contribution to journalArticle

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