Spectrofluorimetric Determination of Individual Bile Acids in Biological Fluids: Duodenal Content and Bile

T. Fehér, J. Papp, M. H. Kazik

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A micromethod is described for the separation and quantitative determination of individual bile acids in duodenal content and bile. After a mild saponification procedure for splitting the taurine and glycine conjugates, the products are separated by thin-layer chromatography on silicagel layer with the solvent system iso-octane-ethyl acetate-acetic acid (60 ml + 35 ml + 5 ml) and determined by spectrofluorirrietry. A series of control experiments has been performed on the validation of the procedure. Values obtained for human subjects or experimental applications of the method are presented.

Original languageEnglish
Pages (from-to)376-380
Number of pages5
JournalClinical Chemistry and Laboratory Medicine
Volume11
Issue number9
DOIs
Publication statusPublished - 1973

Fingerprint

Saponification
Thin layer chromatography
Taurine
Bile Acids and Salts
Bile
Acetic Acid
Glycine
Fluids
Experiments
Thin Layer Chromatography
octane
ethyl acetate

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Spectrofluorimetric Determination of Individual Bile Acids in Biological Fluids : Duodenal Content and Bile. / Fehér, T.; Papp, J.; Kazik, M. H.

In: Clinical Chemistry and Laboratory Medicine, Vol. 11, No. 9, 1973, p. 376-380.

Research output: Contribution to journalArticle

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