Spectrofluorimetric Determination of Individual Bile Acids in Biological Fluids: Duodenal Content and Bile

T. Fehér, J. Papp, M. H. Kazik

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A micromethod is described for the separation and quantitative determination of individual bile acids in duodenal content and bile. After a mild saponification procedure for splitting the taurine and glycine conjugates, the products are separated by thin-layer chromatography on silicagel layer with the solvent system iso-octane-ethyl acetate-acetic acid (60 ml + 35 ml + 5 ml) and determined by spectrofluorirrietry. A series of control experiments has been performed on the validation of the procedure. Values obtained for human subjects or experimental applications of the method are presented.

Original languageEnglish
Pages (from-to)376-380
Number of pages5
JournalClinical Chemistry and Laboratory Medicine
Volume11
Issue number9
DOIs
Publication statusPublished - Jan 1 1973

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

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