Spectral properties of a long‐wavelength absorbing form of protochlorophyll in seeds of Cyclanthera explodens

CHRISTER SUNDQVIST, HANS RYBERG, B. Böddi, FERENC LÁNG

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The in vivo absorbance spectrum of the inner seed coat of Cyclanthera explodens Naud. showed a main peak in the red region at 671 nm and a weak shoulder at about 640 nm. The pigments were extracted with acetone. separated by paper chromatography and analysed spectrophotometrically. The only detectable pigment was protochlorophyll. The in vivo fluorsecence emission spectra had two main peaks, one at 632 and one at 691 nm. The relation between the two peaks was changed when the exvcitation wavelength was altered from 440 to 460 nm. Excitation at 420 nm gave an additional fluorescence emission peak at 595 nm. These data indicate the presence of at least three forms of protochlorophyll in the Cyclantera seed coat. The spectrum of circular dichroism had a very intence and characteristic signal in the red region with a negative asymmetrical Cotton effect (664 (+), 669 (0) and 687 (−) nm). This indicates that at least one of the protochlorophyll forms is present in a more or less crystalline form.

Original languageEnglish
Pages (from-to)297-301
Number of pages5
JournalPhysiologia Plantarum
Volume48
Issue number2
DOIs
Publication statusPublished - Jan 1 1980

Fingerprint

Cyclanthera
protochlorophyll
Seeds
seeds
pigments
circular dichroism spectroscopy
Paper Chromatography
paper chromatography
Circular Dichroism
Acetone
shoulders
acetone
absorbance
wavelengths
cotton
Fluorescence
fluorescence
seed coat

Keywords

  • absorption spectra
  • circular dichroism spectra
  • Cyclanthera
  • fluorescence spectra
  • protochlorophyll
  • protochloropyll aggregates
  • seed coat

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science
  • Cell Biology

Cite this

Spectral properties of a long‐wavelength absorbing form of protochlorophyll in seeds of Cyclanthera explodens. / SUNDQVIST, CHRISTER; RYBERG, HANS; Böddi, B.; LÁNG, FERENC.

In: Physiologia Plantarum, Vol. 48, No. 2, 01.01.1980, p. 297-301.

Research output: Contribution to journalArticle

SUNDQVIST, CHRISTER ; RYBERG, HANS ; Böddi, B. ; LÁNG, FERENC. / Spectral properties of a long‐wavelength absorbing form of protochlorophyll in seeds of Cyclanthera explodens. In: Physiologia Plantarum. 1980 ; Vol. 48, No. 2. pp. 297-301.
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N2 - The in vivo absorbance spectrum of the inner seed coat of Cyclanthera explodens Naud. showed a main peak in the red region at 671 nm and a weak shoulder at about 640 nm. The pigments were extracted with acetone. separated by paper chromatography and analysed spectrophotometrically. The only detectable pigment was protochlorophyll. The in vivo fluorsecence emission spectra had two main peaks, one at 632 and one at 691 nm. The relation between the two peaks was changed when the exvcitation wavelength was altered from 440 to 460 nm. Excitation at 420 nm gave an additional fluorescence emission peak at 595 nm. These data indicate the presence of at least three forms of protochlorophyll in the Cyclantera seed coat. The spectrum of circular dichroism had a very intence and characteristic signal in the red region with a negative asymmetrical Cotton effect (664 (+), 669 (0) and 687 (−) nm). This indicates that at least one of the protochlorophyll forms is present in a more or less crystalline form.

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