Spectral components of cytosolic [Ca2+] spiking in neurons

Julianna Kardos, Nóra Szilégyi, Gábor Juhász, Bo Belhage, Arne Schousboe

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We show here, by means of evolutionary spectral analysis and synthesis of cytosolic Ca2+ ([Ca2+](c)) spiking observed at the single cell level using digital imaging fluorescence microscopy of fura-2-loaded mouse cerebellar granule cells in culture, that [Ca2+](c) spiking can be resolved into evolutionary spectra of a characteristic set of frequencies. Non- delayed small spikes on top of sustained [Ca2+](c) were synthesized by a main component frequency, 0.132 ± 0.012 Hz, showing its maximal amplitude in phase with the start of depolarization (25 mM KCl) combined with caffeine (10 mM) application. Delayed complex responses of large [Ca2+](c) spiking observed in cells from a different set of cultures were synthesized by a set of frequencies within the range 0.018-0.117 Hz. Differential frequency patterns are suggested as characteristics of the [Ca2+](c) spiking responses of neurons under different conditions.

Original languageEnglish
Pages (from-to)721-724
Number of pages4
JournalNeuroreport
Volume9
Issue number4
DOIs
Publication statusPublished - Mar 9 1998

Keywords

  • Caffeine
  • Cerebellar granule cells
  • Spectral components
  • [Ca](c) spiking

ASJC Scopus subject areas

  • Neuroscience(all)

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