Specific von Hippel-Lindau protein expression of clear cell renal cell carcinoma with "immunogenic" features

T. Magyarlaki, I. Buzogány, J. Nagy

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2 Citations (Scopus)

Abstract

Human clear cell renal cell carcinoma (CCRCC) is characterized by specific von Hippel-Lindau (VHL) gene alterations and "immunogenic" features. In the present study, the immunohistochemical expression of the von Hippel-Lindau gene protein (pVHL) was compared with the presence of major histocompatibility complex (MHC I-II), tumor infiltrating lymphocytes (TIL) and tumoral immune complexes (TIC) in CCRCC. Native tumor tissues of 132 RCC patients (95 with the common clear cell subtype), diagnosed according to the Heidelberg classification, were obtained for immunohistochemistry. Tumor stainings with pVHL, MHC I-II and tumor infiltrating lymphocytes (T and B lymphocytes, monocytes) were detected by immunoperoxidase methods using monoclonal antibodies. Tumoral immune complexes (IgG, IgA, IgM and C1q, C3 complement proteins) were visualized by fluorescent polyclonal antibodies. Immune stainings were semiquantitatively evaluated. Specificity and sensitivity of these markers in relation to the common histological subtype of RCC (CCRCC) were calculated. CCRCC was characterized by specific pVHL expression. At the same time, CCRCC was associated with constitutional MHC I-II expression and highly specific degree of TIL and TIC. It is concluded that specific pVHL expression of CCRCC is frequently associated with "immunogenic" features. Immunohistochemical analysis aims the initial tumor staging of RCC patients to achieve better patient selection for immunotherapy. However, the association of pVHL expression with the "immunogenic" CCRCC is statistically relevant, the mechanism and its clinical relevance in immunotherapy still remains to be tested.

Original languageEnglish
Pages (from-to)42-45
Number of pages4
JournalPathology and Oncology Research
Volume7
Issue number1
Publication statusPublished - 2001

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Renal Cell Carcinoma
Tumor-Infiltrating Lymphocytes
Antigen-Antibody Complex
Major Histocompatibility Complex
Proteins
Immunotherapy
Staining and Labeling
Neoplasm Staging
Immunoglobulin A
Patient Selection
Immunoglobulin M
Monocytes
Neoplasms
Complement System Proteins
B-Lymphocytes
Immunoglobulin G
Immunohistochemistry
Monoclonal Antibodies
T-Lymphocytes
Sensitivity and Specificity

Keywords

  • Clear cell renal cell carcinoma
  • Immune complexes
  • Tumor infiltrating lymphocytes
  • Von Hippel-Lindau protein

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Pathology and Forensic Medicine

Cite this

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title = "Specific von Hippel-Lindau protein expression of clear cell renal cell carcinoma with {"}immunogenic{"} features",
abstract = "Human clear cell renal cell carcinoma (CCRCC) is characterized by specific von Hippel-Lindau (VHL) gene alterations and {"}immunogenic{"} features. In the present study, the immunohistochemical expression of the von Hippel-Lindau gene protein (pVHL) was compared with the presence of major histocompatibility complex (MHC I-II), tumor infiltrating lymphocytes (TIL) and tumoral immune complexes (TIC) in CCRCC. Native tumor tissues of 132 RCC patients (95 with the common clear cell subtype), diagnosed according to the Heidelberg classification, were obtained for immunohistochemistry. Tumor stainings with pVHL, MHC I-II and tumor infiltrating lymphocytes (T and B lymphocytes, monocytes) were detected by immunoperoxidase methods using monoclonal antibodies. Tumoral immune complexes (IgG, IgA, IgM and C1q, C3 complement proteins) were visualized by fluorescent polyclonal antibodies. Immune stainings were semiquantitatively evaluated. Specificity and sensitivity of these markers in relation to the common histological subtype of RCC (CCRCC) were calculated. CCRCC was characterized by specific pVHL expression. At the same time, CCRCC was associated with constitutional MHC I-II expression and highly specific degree of TIL and TIC. It is concluded that specific pVHL expression of CCRCC is frequently associated with {"}immunogenic{"} features. Immunohistochemical analysis aims the initial tumor staging of RCC patients to achieve better patient selection for immunotherapy. However, the association of pVHL expression with the {"}immunogenic{"} CCRCC is statistically relevant, the mechanism and its clinical relevance in immunotherapy still remains to be tested.",
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author = "T. Magyarlaki and I. Buzog{\'a}ny and J. Nagy",
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AU - Buzogány, I.

AU - Nagy, J.

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N2 - Human clear cell renal cell carcinoma (CCRCC) is characterized by specific von Hippel-Lindau (VHL) gene alterations and "immunogenic" features. In the present study, the immunohistochemical expression of the von Hippel-Lindau gene protein (pVHL) was compared with the presence of major histocompatibility complex (MHC I-II), tumor infiltrating lymphocytes (TIL) and tumoral immune complexes (TIC) in CCRCC. Native tumor tissues of 132 RCC patients (95 with the common clear cell subtype), diagnosed according to the Heidelberg classification, were obtained for immunohistochemistry. Tumor stainings with pVHL, MHC I-II and tumor infiltrating lymphocytes (T and B lymphocytes, monocytes) were detected by immunoperoxidase methods using monoclonal antibodies. Tumoral immune complexes (IgG, IgA, IgM and C1q, C3 complement proteins) were visualized by fluorescent polyclonal antibodies. Immune stainings were semiquantitatively evaluated. Specificity and sensitivity of these markers in relation to the common histological subtype of RCC (CCRCC) were calculated. CCRCC was characterized by specific pVHL expression. At the same time, CCRCC was associated with constitutional MHC I-II expression and highly specific degree of TIL and TIC. It is concluded that specific pVHL expression of CCRCC is frequently associated with "immunogenic" features. Immunohistochemical analysis aims the initial tumor staging of RCC patients to achieve better patient selection for immunotherapy. However, the association of pVHL expression with the "immunogenic" CCRCC is statistically relevant, the mechanism and its clinical relevance in immunotherapy still remains to be tested.

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