Specific syndecan-1 domains regulate mesenchymal tumor cell adhesion, motility and migration

Fang Zong, Eleni Fthenou, Filip Mundt, Tünde Szatmári, I. Kovalszky, L. Szilák, David Brodin, George Tzanakakis, Anders Hjerpe, Katalin Dobra

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Background: Syndecans are proteoglycans whose core proteins have a short cytoplasmic domain, a transmembrane domain and a large N-terminal extracellular domain possessing glycosaminoglycan chains. Syndecans are involved in many important cellular processes. Our recent publications have demonstrated that syndecan-1 translocates into the nucleus and hampers tumor cell proliferation. In the present study, we aimed to investigate the role of syndecan-1 in tumor cell adhesion and migration, with special focus on the importance of its distinct protein domains, to better understand the structure-function relationship of syndecan-1 in tumor progression. Methodology/Principal Findings: We utilized two mesenchymal tumor cell lines which were transfected to stably overexpress full-length syndecan-1 or truncated variants: the 78 which lacks the extracellular domain except the DRKE sequence proposed to be essential for oligomerization, the 77 which lacks the whole extracellular domain, and the RMKKK which serves as a nuclear localization signal. The deletion of the RMKKK motif from full-length syndecan-1 abolished the nuclear translocation of this proteoglycan. Various bioassays for cell adhesion, chemotaxis, random movement and wound healing were studied. Furthermore, we performed gene microarray to analyze the global gene expression pattern influenced by syndecan-1. Both full-length and truncated syndecan-1 constructs decrease tumor cell migration and motility, and affect cell adhesion. Distinct protein domains have differential effects, the extracellular domain is more important for promoting cell adhesion, while the transmembrane and cytoplasmic domains are sufficient for inhibition of cell migration. Cell behavior seems to depend also on the nuclear translocation of syndecan-1. Many genes are differentially regulated by syndecan-1 and a number of genes are actually involved in cell adhesion and migration. Conclusions/Significance: Our results demonstrate that syndecan-1 regulates mesenchymal tumor cell adhesion and migration, and different domains have differential effects. Our study provides new insights into better understanding of the role of syndecans in tumor progression.

Original languageEnglish
Article numbere14816
JournalPLoS One
Volume6
Issue number6
DOIs
Publication statusPublished - 2011

Fingerprint

Syndecan-1
Cell adhesion
cell movement
cell adhesion
Cell Adhesion
Cell Movement
Tumors
Syndecans
Neoplasms
proteoglycans
Genes
Cells
Proteoglycans
nuclear localization signals
neoplasms
genes
proteins
chemotaxis
glycosaminoglycans
structure-activity relationships

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Specific syndecan-1 domains regulate mesenchymal tumor cell adhesion, motility and migration. / Zong, Fang; Fthenou, Eleni; Mundt, Filip; Szatmári, Tünde; Kovalszky, I.; Szilák, L.; Brodin, David; Tzanakakis, George; Hjerpe, Anders; Dobra, Katalin.

In: PLoS One, Vol. 6, No. 6, e14816, 2011.

Research output: Contribution to journalArticle

Zong, F, Fthenou, E, Mundt, F, Szatmári, T, Kovalszky, I, Szilák, L, Brodin, D, Tzanakakis, G, Hjerpe, A & Dobra, K 2011, 'Specific syndecan-1 domains regulate mesenchymal tumor cell adhesion, motility and migration', PLoS One, vol. 6, no. 6, e14816. https://doi.org/10.1371/journal.pone.0014816
Zong, Fang ; Fthenou, Eleni ; Mundt, Filip ; Szatmári, Tünde ; Kovalszky, I. ; Szilák, L. ; Brodin, David ; Tzanakakis, George ; Hjerpe, Anders ; Dobra, Katalin. / Specific syndecan-1 domains regulate mesenchymal tumor cell adhesion, motility and migration. In: PLoS One. 2011 ; Vol. 6, No. 6.
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AU - Kovalszky, I.

AU - Szilák, L.

AU - Brodin, David

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