SPECIFIC SUPPRESSOR T CELL FUNCTION IN A PATIENT WITH GRAVES' DISEASE AND HER HEALTHY IDENTICAL TWIN

C. Balázs, VALERIA STENSZKY, L. KOZMA, NADIR R. FARID

Research output: Contribution to journalArticle

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Abstract

Immunoregulatory defects have been suggested in autoimmune disorders including GRAVES' disease. The finding that Concanavalin A‐induced suppressor T cell function was sub‐optimal in GRAVES' disease has been disputed; a restricted defect in TSH‐receptor antigen‐specific suppressor cells has instead been proposed by Okita et al. (1980). To explore this further, we studied both specific and non‐specific suppressor cell function in a pair of HLA identical twins, one of whom had GRAVES' disease. By contrast to the euthyroid healthy twin and 10 healthy controls (612 cpm/106 cells) the patient's mononuclear cells (MNCs) incorporated more {3H}‐thymidine (7365 cpm/106 cells) in response to thyroid membrane antigen (TMA). Removal of glass‐adherent cells before addition of antigen increased {3H}‐uptake by cells from the healthy twin to 1808 cpm but reduced those from the GRAVES' twin to 3411 cpm. The influence of MNCs cultured with Con A or TMA for 24 h upon {3H}‐thymidine uptake by 2 × 106 indicator cells triggered by Con A for 72 h or TMA for 96 h was taken as a measure of non‐specific and specific suppressor cell function respectively. Both Con A and TMA induced suppressor cells were reduced, the latter to a more marked degree, in the patient compared to the healthy twin; mixing of MNCs from patient and healthy twin in a 1:1 ratio improved the patient suppressor cell function. When the patient's MNCs triggered for 24 h with Con A were mixed in a 1:1 ratio with her fresh MNCs and TMA, less blast transformation was found compared to an equal number of fresh cells (3H‐thymidine uptake 3250 vs 7365 cpm/106). Similarly, preincubated cells from the healthy twin had greater suppressive effect (1820 cpm/106 cells). We conclude that (I) the HLA identical healthy twin has TMA autoreactive lymphocytes regulated by adherent regulatory cells; (2) the increased ratio of helper/suppressor cells in the adherent cell population in the patient leads to a decrease of {3H}incorporation upon their removal; (3) in the patient, the specific suppressor cell defect is more severe than the non‐specific defect; (4) lack of specific TMA induced triggering may be the critical immunoregulatory defect in Graves' disease.

Original languageEnglish
Pages (from-to)683-693
Number of pages11
JournalClinical Endocrinology
Volume20
Issue number6
DOIs
Publication statusPublished - 1984

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Monozygotic Twins
Graves Disease
T-Lymphocytes
Thyroid Gland
Antigens
Membranes
Thymidine

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Medicine(all)

Cite this

SPECIFIC SUPPRESSOR T CELL FUNCTION IN A PATIENT WITH GRAVES' DISEASE AND HER HEALTHY IDENTICAL TWIN. / Balázs, C.; STENSZKY, VALERIA; KOZMA, L.; FARID, NADIR R.

In: Clinical Endocrinology, Vol. 20, No. 6, 1984, p. 683-693.

Research output: Contribution to journalArticle

Balázs, C. ; STENSZKY, VALERIA ; KOZMA, L. ; FARID, NADIR R. / SPECIFIC SUPPRESSOR T CELL FUNCTION IN A PATIENT WITH GRAVES' DISEASE AND HER HEALTHY IDENTICAL TWIN. In: Clinical Endocrinology. 1984 ; Vol. 20, No. 6. pp. 683-693.
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