Specific radioimmunoassay of alpha-melanocyte-stimulating hormone in rat plasma

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Abstract

A sensitive, specific and reproducible radioimmunoassay was developed for the measurement of alpha-melanocyte-stimulating hormone (α-MSH) in the blood plasma of rat. The assay method is based on a sensitive antiserum raised against α-MSH in rabbit. The serum is highly specific to α-MSH; a PHLC study of an extract of rat plasma showed that the immunoreactivity was given by α-MSH. The basal level of α-MSH, measured after a simple extraction with ethanol, was found to be 168.3 ± 16.3 pg/ml (X̄ ± SEM). Ether and lysine-vasopressin appeared to be potent stimuli for the peripheral release of α-MSH.

Original languageEnglish
Pages (from-to)45-51
Number of pages7
JournalExperimental and Clinical Endocrinology
Volume93
Issue number1
Publication statusPublished - 1989

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Melanocyte-Stimulating Hormones
alpha-MSH
Radioimmunoassay
Lypressin
Ether
Immune Sera
Ethanol
Rabbits
Serum

ASJC Scopus subject areas

  • Endocrinology

Cite this

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abstract = "A sensitive, specific and reproducible radioimmunoassay was developed for the measurement of alpha-melanocyte-stimulating hormone (α-MSH) in the blood plasma of rat. The assay method is based on a sensitive antiserum raised against α-MSH in rabbit. The serum is highly specific to α-MSH; a PHLC study of an extract of rat plasma showed that the immunoreactivity was given by α-MSH. The basal level of α-MSH, measured after a simple extraction with ethanol, was found to be 168.3 ± 16.3 pg/ml (X̄ ± SEM). Ether and lysine-vasopressin appeared to be potent stimuli for the peripheral release of α-MSH.",
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AU - Julesz, J.

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AB - A sensitive, specific and reproducible radioimmunoassay was developed for the measurement of alpha-melanocyte-stimulating hormone (α-MSH) in the blood plasma of rat. The assay method is based on a sensitive antiserum raised against α-MSH in rabbit. The serum is highly specific to α-MSH; a PHLC study of an extract of rat plasma showed that the immunoreactivity was given by α-MSH. The basal level of α-MSH, measured after a simple extraction with ethanol, was found to be 168.3 ± 16.3 pg/ml (X̄ ± SEM). Ether and lysine-vasopressin appeared to be potent stimuli for the peripheral release of α-MSH.

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