Specific hydrolysis and accumulation of antiproliferative lignans in the fruit of Leuzea carthamoides (Willd.) DC.

Anna Sólyomváry, Zsolt Mervai, Ibolya Molnár-Perl, Imre Boldizsár

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Dibenzylbutyrolactone-type lignan glycosides (tracheloside and carthamoside), their aglycones (trachelogenin and carthamogenin) and feruloyl-serotonin isomers were determined in the fruits of Leuzea carthamoides by using LC-UV, LC-MS/MS and GC-MS techniques. The composition of the embryo and wall parts of the fruits was analysed before and after their hydrolysis. As a result of these studies, fruit part-specific accumulation of lignan glycosides and feruloyl-serotonins were confirmed, demonstrating that the embryo contains a high amount of lignan glycosides (tracheloside 32.9 mg/g, carthamoside 45.3 mg/g), while the wall part of the fruit accumulates feruloyl-serotonins (63.0 mg/g). Enzymatic hydrolysis of the embryo resulted in the quantitative transformation of lignan glycosides into their corresponding aglycones, allowing selective isolation of trachelogenin and carthamogenin. These aglycones were subjected to an antiproliferative study against the SW480 colon adenocarcinoma cell line. In this test, moderate activity of carthamogenin and a significant effect of trachelogenin were demonstrated in a concentration range of 22-185 M.

Original languageEnglish
Pages (from-to)732-739
Number of pages8
JournalNatural Product Research
Volume28
Issue number10
DOIs
Publication statusPublished - May 19 2014

Keywords

  • Analysis of lignans
  • Antiproliferative activity
  • Arctigenin
  • Carthamogenin
  • Leuzea
  • Trachelogenin

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Plant Science
  • Organic Chemistry

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