Specific amino acids moderate the effects of Ni2+ on the testosterone production of mouse Leydig cells in vitro

Z. Forgács, Zsolt Némethy, Csaba Révész, Péter Lázár

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The purpose of this investigation was to study the effectiveness of two nickel-binding amino acids, histidine (His) and cysteine (Cys), to prevent the inhibitory action of Ni2+ on testosterone (T) production by mouse primary Leydig cell culture. The maximal human chorionic gonadotropin (hCG)-stimulated T response was measured by radioimmunoassay (RIA) in the culture media. Three types of experiments were performed. In a concentration-response study, Ni2+ (62.5 to 1000 μM) was added to the cells simultaneously with equimolar or twice the equimolar concentrations of His or Cys and the cultures were maintained for 48 h. Nickel-induced reduction in T production was completely prevented by equimolar concentrations of His at Ni2+ concentrations of 125, 250, and 500 μM; equimolar or twice the equimolar concentrations of His were only partially effective at 1000 μM Ni2+. Protective action of Cys was complete only at the lowest concentration of Ni2+ (125 μM). In a second series, the cells were incubated for various times (0.5 to 48 h) with 1000 μM Ni2+ in the presence of 2000 μM His or Cys. Increasing the time of incubation, the protective effect of both amino acids against Ni2+ was reduced. In a third series, attempts were made to reverse the action of 1000 μM Ni2+ after incubation with cells for various times (0.5 to 24 h), followed by exposure to 2000 μM His or Cys. Cell cultures were maintained for 48 h. A partial recovery of hCG-stimulated T production could be observed only if the amino acid was added not later than 4 h after the metal. This time was also required to elicit the T depression produced by Ni2+. Administration of either His or Cys at later times had no effect. Our results show that both His and Cys are able to moderate the effects of Ni2+ on Leydig cell T production, depending on the concentration of this metal ion, as well as on amino acid. However, at higher Ni2+ concentrations the complete protection by His or Cys is only temporary. Administration of these amino acids after the Ni2+-produced decrease in T production was not able to reverse the process.

Original languageEnglish
Pages (from-to)349-358
Number of pages10
JournalJournal of Toxicology and Environmental Health - Part A
Volume62
Issue number5
DOIs
Publication statusPublished - Mar 9 2001

Fingerprint

Leydig Cells
testosterone
Histidine
Testosterone
Amino acids
Cysteine
amino acid
Amino Acids
Cell culture
nickel
incubation
Nickel
Chorionic Gonadotropin
metal
Metal ions
Metals
In Vitro Techniques
effect
Primary Cell Culture
Recovery

ASJC Scopus subject areas

  • Environmental Science(all)
  • Environmental Chemistry
  • Public Health, Environmental and Occupational Health
  • Pollution
  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Specific amino acids moderate the effects of Ni2+ on the testosterone production of mouse Leydig cells in vitro. / Forgács, Z.; Némethy, Zsolt; Révész, Csaba; Lázár, Péter.

In: Journal of Toxicology and Environmental Health - Part A, Vol. 62, No. 5, 09.03.2001, p. 349-358.

Research output: Contribution to journalArticle

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