Species specific IDMS for accurate quantification of carboplatin in urine by LC-ESI-TOFMS and LC-ICP-QMS

G. Koellensperger, Zs Stefanka, K. Meelich, M. Galanski, B. K. Keppler, G. Stingeder, S. Hann

Research output: Contribution to journalArticle

19 Citations (Scopus)


For the first time, 194Pt isotopically enriched carboplatin has been synthesized, serving for species specific isotope dilution analysis. Carboplatin was determined in urine, implementing both LC-ICP-QMS and LC-ESI-TOFMS. Hence, a separation method was applied, compatible with both ICP-QMS and ESI-MS detection. IDMS quantification was evaluated in terms of measurement uncertainty, comparing the two mass spectrometric detection methods. The procedural limits of detection of HPLC-ICP-QMS and HPLC-ESI-TOFMS were 0.1 ng g -1 and 15 ng g -1, respectively. Both methodologies were successfully applied to the quantification of carboplatin in the urine of a chemotherapy patient. The obtained carboplatin concentrations agreed within their uncertainties. Uncertainty budgeting calculations revealed that the accuracy of quantification was primarily limited by the precision of isotope ratio measurement, governing the determination of the ratios R x, R y and R b of the IDMS equation. For ICP-QMS, a total combined uncertainty of 5.7% (coverage factor 2) was assessed. In accordance to the impaired precision of blend ratio determination in ESI-TOFMS (precision of 5% compared to 1% in ICP-QMS), this method revealed a total combined uncertainty of 23%.

Original languageEnglish
Pages (from-to)29-36
Number of pages8
JournalJournal of analytical atomic spectrometry
Issue number1
Publication statusPublished - Jan 1 2008

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy

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