Speciation of inorganic selenium and selenoamino acids by an HPLC-UV-HG-AFS system

I. Ipolyi, W. Corns, P. Stockwell, P. Fodor

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For the on-line speciation of selenocystine (SeCys), selenomethionine (SeMet), selenoethionine (SeEt), selenite (Se(IV)) and selenate (Se(VI)), a high-performance liquid chromatography-UV irradiation-hydride generation-atomic fluorescence spectrometric method is described. Separation was carried out on a conventional reversed-phase C18 column modified with didodecyldimethylammonium bromide with gradient elution applying two concentrations of ammonium acetate as the mobile phase. UV irradiation and hydride generation parameters were optimized. The obtained detection limits for SeCys, SeMet, SeEt, Se(IV) and Se(VI) were 0.31, 0.43, 0.7, 0.44 and 0.32 ng ml-1, respectively, using a 100-μl loop. The method was tested with spiked mineral water and two volunteers' urine samples.

Original languageEnglish
Pages (from-to)167-172
Number of pages6
JournalJournal of Automated Methods and Management in Chemistry
Issue number6
Publication statusPublished - Jan 1 2001


ASJC Scopus subject areas

  • Analytical Chemistry
  • Chemical Engineering (miscellaneous)
  • Instrumentation
  • Computer Science Applications

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