Specialization of CDC27 function in the Arabidopsis thaliana anaphase-promoting complex (APC/C)

José M. Pérez-Pérez, Olivier Serralbo, Marleen Vanstraelen, Cristina González, Marie Claire Criqui, Pascal Genschik, É. Kondorosi, Ben Scheres

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

To investigate the specialization of the two Arabidopsis CDC27 subunits in the anaphase-promoting complex (APC/C), we analyzed novel alleles of HBT/CDC27B and CDC27A, and characterized the expression of complementing HOBBIT (HBT) protein fusions in plant meristems and during the cell cycle. In contrast to other APC/C mutants, which are gametophytic lethal, phenotypes of weak and null hbt alleles indicate a primary role in the control of post-embryonic cell division and cell elongation, whereas cdc27a nulls are phenotypically indistinguishable from the wild type. However, cdc27a hbt double-mutant gametes are non-viable, indicating a redundant requirement for both CDC27 subunits during gametogenesis. Yeast-two-hybrid and pulldown studies with APC/C components suggest that the two Arabidopsis CDC27 subunits participate in several complexes that are differentially required during plant development. Loss-of-function analysis, as well as cyclin B reporter protein accumulation, indicates a conserved role for the plant APC/C in controlling mitotic progression and cell differentiation during the entire life cycle.

Original languageEnglish
Pages (from-to)78-89
Number of pages12
JournalPlant Journal
Volume53
Issue number1
DOIs
Publication statusPublished - Jan 2008

Fingerprint

Anaphase-Promoting Complex-Cyclosome
Arabidopsis
Arabidopsis thaliana
Alleles
Gametogenesis
Cyclin B
mutants
Plant Development
Meristem
null alleles
cyclins
gametogenesis
Life Cycle Stages
lethal genes
Germ Cells
meristems
cell differentiation
Cell Division
plant development
cell division

Keywords

  • Cell cycle
  • Checkpoint
  • Cyclosome
  • HOBBIT
  • Root development

ASJC Scopus subject areas

  • Plant Science

Cite this

Pérez-Pérez, J. M., Serralbo, O., Vanstraelen, M., González, C., Criqui, M. C., Genschik, P., ... Scheres, B. (2008). Specialization of CDC27 function in the Arabidopsis thaliana anaphase-promoting complex (APC/C). Plant Journal, 53(1), 78-89. https://doi.org/10.1111/j.1365-313X.2007.03312.x

Specialization of CDC27 function in the Arabidopsis thaliana anaphase-promoting complex (APC/C). / Pérez-Pérez, José M.; Serralbo, Olivier; Vanstraelen, Marleen; González, Cristina; Criqui, Marie Claire; Genschik, Pascal; Kondorosi, É.; Scheres, Ben.

In: Plant Journal, Vol. 53, No. 1, 01.2008, p. 78-89.

Research output: Contribution to journalArticle

Pérez-Pérez, JM, Serralbo, O, Vanstraelen, M, González, C, Criqui, MC, Genschik, P, Kondorosi, É & Scheres, B 2008, 'Specialization of CDC27 function in the Arabidopsis thaliana anaphase-promoting complex (APC/C)', Plant Journal, vol. 53, no. 1, pp. 78-89. https://doi.org/10.1111/j.1365-313X.2007.03312.x
Pérez-Pérez JM, Serralbo O, Vanstraelen M, González C, Criqui MC, Genschik P et al. Specialization of CDC27 function in the Arabidopsis thaliana anaphase-promoting complex (APC/C). Plant Journal. 2008 Jan;53(1):78-89. https://doi.org/10.1111/j.1365-313X.2007.03312.x
Pérez-Pérez, José M. ; Serralbo, Olivier ; Vanstraelen, Marleen ; González, Cristina ; Criqui, Marie Claire ; Genschik, Pascal ; Kondorosi, É. ; Scheres, Ben. / Specialization of CDC27 function in the Arabidopsis thaliana anaphase-promoting complex (APC/C). In: Plant Journal. 2008 ; Vol. 53, No. 1. pp. 78-89.
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