Specialization of CDC27 function in the Arabidopsis thaliana anaphase-promoting complex (APC/C)

José M. Pérez-Pérez, Olivier Serralbo, Marleen Vanstraelen, Cristina González, Marie Claire Criqui, Pascal Genschik, Eva Kondorosi, Ben Scheres

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Abstract

To investigate the specialization of the two Arabidopsis CDC27 subunits in the anaphase-promoting complex (APC/C), we analyzed novel alleles of HBT/CDC27B and CDC27A, and characterized the expression of complementing HOBBIT (HBT) protein fusions in plant meristems and during the cell cycle. In contrast to other APC/C mutants, which are gametophytic lethal, phenotypes of weak and null hbt alleles indicate a primary role in the control of post-embryonic cell division and cell elongation, whereas cdc27a nulls are phenotypically indistinguishable from the wild type. However, cdc27a hbt double-mutant gametes are non-viable, indicating a redundant requirement for both CDC27 subunits during gametogenesis. Yeast-two-hybrid and pulldown studies with APC/C components suggest that the two Arabidopsis CDC27 subunits participate in several complexes that are differentially required during plant development. Loss-of-function analysis, as well as cyclin B reporter protein accumulation, indicates a conserved role for the plant APC/C in controlling mitotic progression and cell differentiation during the entire life cycle.

Original languageEnglish
Pages (from-to)78-89
Number of pages12
JournalPlant Journal
Volume53
Issue number1
DOIs
Publication statusPublished - Jan 1 2008

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Keywords

  • Cell cycle
  • Checkpoint
  • Cyclosome
  • HOBBIT
  • Root development

ASJC Scopus subject areas

  • Genetics
  • Plant Science
  • Cell Biology

Cite this

Pérez-Pérez, J. M., Serralbo, O., Vanstraelen, M., González, C., Criqui, M. C., Genschik, P., Kondorosi, E., & Scheres, B. (2008). Specialization of CDC27 function in the Arabidopsis thaliana anaphase-promoting complex (APC/C). Plant Journal, 53(1), 78-89. https://doi.org/10.1111/j.1365-313X.2007.03312.x