Spatial and temporal changes of bacterial communities inhabiting the well waters of Harkány spa

A. Borsodi, Roland Miseta, Márton Palatinszky, Judit Makk, K. Márialigeti

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

In this study, changes in the bacterial community composition of the well waters of Harkány Spa were examined. Physical and chemical properties of mixing subsurface cold and thermal karst waters were correlated to shifts in bacterial community structures analyzed by denaturing gradient gel electrophoresis (DGGE) and principal component analysis (PCA). In addition, mineral components of the pellets were investigated by scanning electron microscopy. Samples from the effluent waters of Büdöstapolca I and II, Matty and Thermal VI wells were taken seasonally in 2007 and 2008. The comparison of the results of DGGE and PCA analyses showed that bacterial communities from the Büdöstapolca wells were distinct from those of Matty and Thermal VI, but seasonal changes were not detected. According to the phylogenetic analysis of the excised DGGE bands, presence of chemolithotrophic Proteobacteria (Thiobacillus, Thiothrix, and distant relatives of Sulfurospirillum) were typical in the Büdöstapolca wells, while members of Actinobacteria (Plantibacter, Actinobacterium, Microbacterium) and Firmicutes (Planococcus) were characteristic to the Matty and Thermal VI wells. In the pellets pyrite framboid crystals were observed by electron microscopy, which are minerals known to be biologically induced by dissimilatory iron- and sulfur (sulfate)-reducing bacteria.

Original languageEnglish
Pages (from-to)329-343
Number of pages15
JournalActa Microbiologica et Immunologica Hungarica
Volume60
Issue number3
DOIs
Publication statusPublished - Sep 1 2013

Fingerprint

Denaturing Gradient Gel Electrophoresis
Hot Temperature
Water
Principal Component Analysis
Thiothrix
Minerals
Sulfur-Reducing Bacteria
Thiobacillus
Bacterial Structures
Proteobacteria
Actinobacteria
Electron Scanning Microscopy
Sulfates
Electron Microscopy
Iron

Keywords

  • bacterial communities
  • denaturing gradient gel electrophoresis
  • electron microscopy
  • framboid
  • karst wells
  • principal component analysis

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Medicine(all)

Cite this

Spatial and temporal changes of bacterial communities inhabiting the well waters of Harkány spa. / Borsodi, A.; Miseta, Roland; Palatinszky, Márton; Makk, Judit; Márialigeti, K.

In: Acta Microbiologica et Immunologica Hungarica, Vol. 60, No. 3, 01.09.2013, p. 329-343.

Research output: Contribution to journalArticle

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