Soybean nodule autoregulation receptor kinase phosphorylates two kinase-associated protein phosphatases in vitro

Akira Miyahara, Tripty A. Hirani, Marie Oakes, A. Kereszt, Bostjan Kobe, Michael A. Djordjevic, Peter M. Gresshoff

Research output: Contribution to journalArticle

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Abstract

The NARK (nodule autoregulation receptor kinase) gene, a negative regulator of cell proliferation in nodule primordia in several legumes, encodes a receptor kinase that consists of an extracellular leucine-rich repeat and an intracellular serine/threonine protein kinase domain. The putative catalytic domain of NARK was expressed and purified as a maltose-binding or a glutathione S-transferase fusion protein in Escherichia coli. The recombinant NARK proteins showed autophosphorylation activity in vitro. Several regions of the NARK kinase domain were shown by mass spectrometry to possess phosphoresidues. The kinase-inactive protein K724E failed to autophosphorylate, as did three other proteins corresponding to phenotypically detected mutants defective in whole plant autoregulation of nodulation. A wild-type NARK fusion protein transphosphorylated a kinase-inactive mutant NARK fusion protein, suggesting that it is capable of intermolecular autophosphorylation in vitro. In addition, Ser-861 and Thr-963 in the NARK kinase catalytic domain were identified as phosphorylation sites through site-directed mutagenesis. The genes coding for the kinase-associated protein phosphatases KAPP1 and KAPP2, two putative interacting components of NARK, were isolated. NARK kinase domain phosphorylated recombinant KAPP proteins in vitro. Autophosphorylated NARK kinase domain was, in turn, dephosphorylated by both KAPP1 and KAPP2. Our results suggest a model for signal transduction involving NARK in the control of nodule development.

Original languageEnglish
Pages (from-to)25381-25391
Number of pages11
JournalJournal of Biological Chemistry
Volume283
Issue number37
DOIs
Publication statusPublished - Sep 12 2008

Fingerprint

Phosphoprotein Phosphatases
Soybeans
Homeostasis
Phosphotransferases
Protein Kinases
In Vitro Techniques
Fusion reactions
Proteins
Catalytic Domain
Genes
Maltose
Escherichia coli Proteins
Protein-Serine-Threonine Kinases
Recombinant proteins
Signal transduction
Mutagenesis
Site-Directed Mutagenesis
Phosphorylation
Glutathione Transferase
Recombinant Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Soybean nodule autoregulation receptor kinase phosphorylates two kinase-associated protein phosphatases in vitro. / Miyahara, Akira; Hirani, Tripty A.; Oakes, Marie; Kereszt, A.; Kobe, Bostjan; Djordjevic, Michael A.; Gresshoff, Peter M.

In: Journal of Biological Chemistry, Vol. 283, No. 37, 12.09.2008, p. 25381-25391.

Research output: Contribution to journalArticle

Miyahara, Akira ; Hirani, Tripty A. ; Oakes, Marie ; Kereszt, A. ; Kobe, Bostjan ; Djordjevic, Michael A. ; Gresshoff, Peter M. / Soybean nodule autoregulation receptor kinase phosphorylates two kinase-associated protein phosphatases in vitro. In: Journal of Biological Chemistry. 2008 ; Vol. 283, No. 37. pp. 25381-25391.
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